Brilliant Violet 421™ anti-mouse FOXP3 Antib
- 产品名称：
- Brilliant Violet 421™ anti-mouse FOXP3 Antib
- 产品类别：
- 抗体
- 产品编号：
- 126419
- 产品应用：
- 126419
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
- Application References
(PubMed link indicates BioLegend citation) -
- Ono M, et al. 2007. Nature 446:685.
- Hori S, et al. 2003. Science 299:1057.
- Fontenot JD, et al. 2003 Nature Immunol 4:330.
- Fallarino F, et al. 2009. J. Immunol. 183:6033. PubMed
- Barber A, et al. 2009 J. Immunol. 183:6939. PubMed
- Nakashima H, et al. 2010. J. Immunol. 184:4637. PubMed
- Product Citations
-
- Yao RQ, et al. 2022. Theranostics. 12:4606. PubMed
- Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
- Baram T, et al. 2021. Cells. 10:. PubMed
- Mills C, et al. 2022. Cells. 11:. PubMed
- Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
- Sharma M, et al. 2020. Circ Res. 127:335. PubMed
- Becker W, et al. 2021. J Crohns Colitis. 15:1032. PubMed
- Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
- Xiao Y, et al. 2022. Nat Commun. 13:758. PubMed
- Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
- Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
- Kyburz A, et al. 2017. Clin Exp Allergy. 47:1331. PubMed
- Qadir AS, et al. 2021. iScience. 24:103348. PubMed
- Desai P, et al. 2021. Cell. 184(5):1214-1231.e16. PubMed
- Liu J, et al. 2021. Front Immunol. 12:733808. PubMed
- Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
- Sharma M, et al. 2019. Immunometabolism. 1. PubMed
- Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
- Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
- Tsai S, et al. 2018. Cell Metab. 28:922. PubMed
- RRID
- AB_2565933 (BioLegend Cat. No. 126419)
- Structure
- 50-55 kd protein. Forkhead/winged-helix transcription factor family, contains zinc finger and forkhead domains.
- Distribution
-
Nuclear; expressed in Treg cells.
- Function
- Master regulatory gene in Treg cell development, crucial for immune homeostasis.
- Interaction
- Interacts with DNA
- Cell Type
- Tregs
- Biology Area
- Immunology
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Ono M, et al. 2007. Nature 446:685.
2. Hori S, et al. 2003. Science 299:1057.
3. Fontenot JD, et al. 2003 Nature Immunol 4:330.
- Regulation
- Present at high level in T reg cells. Induced by T cell activation.
- Gene ID
- 20371 View all products for this Gene ID
- UniProt
- View information about FOXP3 on UniProt.org
