Alexa Fluor® 700 anti-mouse FOXP3 Antibody
- 产品名称：
- Alexa Fluor® 700 anti-mouse FOXP3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 126421
- 产品应用：
- 126421
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC -?Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our?True-Nuclear??Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
- Application References
(PubMed link indicates BioLegend citation) -
- Ono M, et al. 2007. Nature 446:685.
- Hori S, et al. 2003. Science 299:1057.
- Fontenot JD, et al. 2003 Nature Immunol 4:330.
- Fallarino F, et al. 2009. J. Immunol. 183:6033. PubMed
- Barber A, et al. 2009 J. Immunol. 183:6939. PubMed
- Nakashima H, et al. 2010. J. Immunol. 184:4637. PubMed
- RRID
- AB_2750492 (BioLegend Cat. No. 126421) AB_2750493 (BioLegend Cat. No. 126422)
- Structure
- 50-55 kd protein. Forkhead/winged-helix transcription factor family, contains zinc finger and forkhead domains.
- Distribution
-
Nuclear; expressed in Treg cells.
- Function
- Master regulatory gene in Treg cell development, crucial for immune homeostasis.
- Interaction
- Interacts with DNA
- Cell Type
- Tregs
- Biology Area
- Immunology
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Ono M, et al. 2007. Nature 446:685.
2. Hori S, et al. 2003. Science 299:1057.
3. Fontenot JD, et al. 2003 Nature Immunol 4:330.
- Regulation
- Present at high level in T reg cells. Induced by T cell activation.
- Gene ID
- 20371 View all products for this Gene ID
- UniProt
- View information about FOXP3 on UniProt.org
