Alexa Fluor® 647 anti-mouse FOXP3 Antibody
- 产品名称：
- Alexa Fluor® 647 anti-mouse FOXP3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 126407
- 产品应用：
- 126407
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
- Application References
(PubMed link indicates BioLegend citation) -
- Ono M, et al. 2007. Nature 446:685.
- Hori S, et al. 2003. Science 299:1057.
- Fontenot JD, et al. 2003 Nature Immunol 4:330.
- Fallarino F, et al. 2009. J. Immunol. 183:6033. PubMed
- Barber A, et al. 2009 J. Immunol. 183:6939. PubMed
- Nakashima H, et al. 2010. J. Immunol. 184:4637. PubMed
- Product Citations
-
- Heindl S, et al. 2021. J Exp Med. 218:. PubMed
- Luo J, et al. 2022. J Nanobiotechnology. 20:228. PubMed
- Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
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- Zhou HF, et al. 2022. Front Pharmacol. 13:778755. PubMed
- Marfil-Garza BA, et al. 2022. Am J Transplant. 22:1101. PubMed
- MacDonald A, et al. 2021. Front Immunol. 12:755995. PubMed
- Feng H, et al. 2020. J Dermatol. 47:147. PubMed
- Willingham SB, et al. 2018. Cancer Immunol Res. 6:1136. PubMed
- Bogie JF, et al. 2020. Ther Adv Chronic Dis. 11:2040622320947378. PubMed
- He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
- Liao T, et al. 2017. Front Immunol. 8:1334. PubMed
- Zhang C, et al. 2022. Nat Commun. 13:3468. PubMed
- Luo J, et al. 2022. J Nanobiotechnology. 20:228. PubMed
- Mender I, et al. 2020. Cancer Cell. 38(3):400-411.e6. PubMed
- Li Y, et al. 2021. Cell Death Dis. 12:1001. PubMed
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- Bao Z, et al. 2020. Ann Transplant. 25:e921287. PubMed
- Molgora M, et al. 2020. Cell. 182:886. PubMed
- Sun F, et al. 2022. Cell Death Dis. 13:181. PubMed
- Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
- Bommareddy PK, et al. 2019. J Biol Methods. 6:2. PubMed
- Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
- Oh J, et al. 2021. Clin Cancer Res. 27:4781. PubMed
- Yue T, et al. 2022. Cell Death Differ. 29:218. PubMed
- Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
- Sheng J, et al. 2021. eLife. 10:00. PubMed
- Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed
- Gray CC, et al. 2022. Front Immunol. 13:861670. PubMed
- Moritsch S, et al. 2022. Oncoimmunology. 11:2127271. PubMed
- Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
- RRID
- AB_1089116 (BioLegend Cat. No. 126407) AB_1089115 (BioLegend Cat. No. 126408)
- Structure
- 50-55 kd protein. Forkhead/winged-helix transcription factor family, contains zinc finger and forkhead domains.
- Distribution
-
Nuclear; expressed in Treg cells.
- Function
- Master regulatory gene in Treg cell development, crucial for immune homeostasis.
- Interaction
- Interacts with DNA
- Cell Type
- Tregs
- Biology Area
- Immunology
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Ono M, et al. 2007. Nature 446:685.
2. Hori S, et al. 2003. Science 299:1057.
3. Fontenot JD, et al. 2003 Nature Immunol 4:330.
- Regulation
- Present at high level in T reg cells. Induced by T cell activation.
- Gene ID
- 20371 View all products for this Gene ID
- UniProt
- View information about FOXP3 on UniProt.org
