Alexa Fluor® 488 anti-mouse FOXP3 Antibody
- 产品名称：
- Alexa Fluor® 488 anti-mouse FOXP3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 126405
- 产品应用：
- 126405
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
IHC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
- Application References
(PubMed link indicates BioLegend citation) -
- Ono M, et al. 2007. Nature 446:685.
- Hori S, et al. 2003. Science 299:1057.
- Fontenot JD, et al. 2003 Nature Immunol 4:330.
- Fallarino F, et al. 2009. J. Immunol. 183:6033. PubMed
- Barber A, et al. 2009 J. Immunol. 183:6939. PubMed
- Nakashima H, et al. 2010. J. Immunol. 184:4637. PubMed
- Product Citations
-
- Ren Z, et al. 2021. EMBO Molecular Medicine. :e14059. PubMed
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- Horikoshi M, et al. 2012. PLoS One. 7:e51215. PubMed
- Newsted D, et al. 2019. Oncoimmunology. 8:e1539613. PubMed
- Freitas JT, et al. 2021. Pigment Cell Melanoma Res. 34:1084. PubMed
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- Chen D, et al. 2020. Cancer Immunol Res. 8:883. PubMed
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- Boyoglu-Barnum S, et al. 2014. J Virol. 88:10569. PubMed
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- Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
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- RRID
- AB_1089114 (BioLegend Cat. No. 126405) AB_1089113 (BioLegend Cat. No. 126406)
- Structure
- 50-55 kd protein. Forkhead/winged-helix transcription factor family, contains zinc finger and forkhead domains.
- Distribution
-
Nuclear; expressed in Treg cells.
- Function
- Master regulatory gene in Treg cell development, crucial for immune homeostasis.
- Interaction
- Interacts with DNA
- Cell Type
- Tregs
- Biology Area
- Immunology
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Ono M, et al. 2007. Nature 446:685.
2. Hori S, et al. 2003. Science 299:1057.
3. Fontenot JD, et al. 2003 Nature Immunol 4:330.
- Regulation
- Present at high level in T reg cells. Induced by T cell activation.
- Gene ID
- 20371 View all products for this Gene ID
- UniProt
- View information about FOXP3 on UniProt.org
