Ultra-LEAF™ Purified anti-mouse CD62L Antibo-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1320	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

C3H/eb mouse B lymphoma 38C-13

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
CyTOF? - Verified
IHC-F, IP, CMCD, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1-3, complement-dependent cytotoxicity⁴, in vivo and in vitro blocking of adhesion^1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections⁶. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 104457-104462).

Application References (PubMed link indicates BioLegend citation)

Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
Olver S, et al. 2006. Cancer Res. 66:571.
Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
Lee JW, et al. 2006. Nature Immunol. 8:181.
Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed

RRID

AB_2832325 (BioLegend Cat. No. 104457) AB_2832326 (BioLegend Cat. No. 104458) AB_2832327 (BioLegend Cat. No. 104459) AB_2832328 (BioLegend Cat. No. 104460) AB_2832329 (BioLegend Cat. No. 104461) AB_2832330 (BioLegend Cat. No. 104462)

Antigen Details

Structure: Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Distribution: Subsets of B and T cells, monocytes, granulocytes, subset of NK cells
Function: Lymphocyte homing to HEV, rolling on activated endothelium
Ligand/Receptor: CD34, GlyCAM-1, MAdCAM-1
Cell Type: B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.
Gene ID: 20343 View all products for this Gene ID
UniProt: View information about CD62L on UniProt.org

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