Purified anti-mouse CD62L (Maxpar® Ready) Anti-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1272	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

C3H/eb mouse B lymphoma 38C-13

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF? - Verified

Recommended Usage

This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1-3, complement-dependent cytotoxicity⁴, in vivo and in vitro blocking of adhesion^1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections⁶. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 104457-104462).

Additional Product Notes

Maxpar? is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
Olver S, et al. 2006. Cancer Res. 66:571.
Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
Lee JW, et al. 2006. Nature Immunol. 8:181.
Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed

Product Citations

Dasgupta D, et al. 2020. Gastroenterology. 159:1487. PubMed
Barvalia M, et al. 2022. Methods Mol Biol. 2508:147. PubMed
Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed

RRID

AB_2562802 (BioLegend Cat. No. 104443)

Antigen Details

Structure: Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Distribution: Subsets of B and T cells, monocytes, granulocytes, subset of NK cells
Function: Lymphocyte homing to HEV, rolling on activated endothelium
Ligand/Receptor: CD34, GlyCAM-1, MAdCAM-1
Cell Type: B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.
Gene ID: 20343 View all products for this Gene ID
UniProt: View information about CD62L on UniProt.org

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