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首页 > 抗体蛋白 > 抗体
Pacific Blue™ anti-mouse CD62L Antibody
产品名称：
Pacific Blue™ anti-mouse CD62L Antibody
产品类别：
抗体
产品编号：
104423
产品应用：
104423
[价格]
规格 价格 库存
25µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/eb mouse B lymphoma 38C-13
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4, in vivo and in vitro blocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 104457-104462).

Application References

(PubMed link indicates BioLegend citation)
  1. Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
  2. Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
  3. Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
  4. Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
  5. Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
  6. Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
  7. Olver S, et al. 2006. Cancer Res. 66:571.
  8. Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
  9. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Lee JW, et al. 2006. Nature Immunol. 8:181.
  12. Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
  13. de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed
Product Citations
  1. Lv H, et al. 2020. Cell Metabolism. 33(1):110-127.e5. PubMed
  2. Freeley S, et al. 2013. Ann Rheum Dis. 72:1053. PubMed
  3. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
  4. Ngamsri KC, et al. 2021. Int J Mol Sci. 22:. PubMed
  5. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  6. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  7. Kurniawan H, et al. 2020. Cell Metabolism. 31(5):920-936. PubMed
  8. Ren G et al. 2017. Molecular cell. 67(6):1049-1058 . PubMed
  9. Jiang Y, et al. 2020. Adv Mater. 32:e2001808. PubMed
  10. Daneshmandi S, et al. 2021. Elife. 10:. PubMed
  11. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  12. Miyauchi E, et al. 2020. Nature. 585:102. PubMed
  13. Sun X, et al. 2021. Nature. 599:673. PubMed
  14. R?mer C et al. 2015. The Journal of Neuroscience. 35(20):7777-94 . PubMed
  15. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
  16. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  17. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  18. Fleskens V, et al. 2019. Cell Rep. 26:3600. PubMed
RRID
AB_493381 (BioLegend Cat. No. 104423) AB_493380 (BioLegend Cat. No. 104424)

Antigen Details

Structure
Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Distribution

Subsets of B and T cells, monocytes, granulocytes, subset of NK cells

Function
Lymphocyte homing to HEV, rolling on activated endothelium
Ligand/Receptor
CD34, GlyCAM-1, MAdCAM-1
Cell Type
B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.

Gene ID
20343 View all products for this Gene ID
UniProt
View information about CD62L on UniProt.org

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