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Brilliant Violet 421™ anti-mouse CD62L Antib
产品名称：
Brilliant Violet 421™ anti-mouse CD62L Antib
产品类别：
抗体
产品编号：
104435
产品应用：
104435
[价格]
规格 价格 库存
125µL ￥ 2112 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/eb mouse B lymphoma 38C-13
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
?g sizes: 0.2 mg/mL
?L sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the ?g size, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. For immunofluorescent staining using the ?l size, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4, in vivo and in vitro blocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 104457-104462).

Application References

(PubMed link indicates BioLegend citation)
  1. Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
  2. Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
  3. Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
  4. Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
  5. Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
  6. Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
  7. Olver S, et al. 2006. Cancer Res. 66:571.
  8. Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
  9. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Lee JW, et al. 2006. Nature Immunol. 8:181.
  12. Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
  13. de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed
Product Citations
  1. Bergot AS, et al. 2020. J Immunol. 204:1787. PubMed
  2. Valanparambil RM, et al. 2017. PLoS Pathog. 13:e1006647. PubMed
  3. Skinner DD, et al. 2022. Front Immunol. 13:931388. PubMed
  4. Aldon Y, et al. 2020. J Immunol. 204:903. PubMed
  5. He Y, et al. 2020. Sci Adv. 6:eaba7589. PubMed
  6. Limon JJ et al. 2019. Cell host & microbe. 25(3):377-388 . PubMed
  7. Wiede F, et al. 2021. Cancer Discov. Online ahead of print. PubMed
  8. Li Z, et al. 2022. Elife. 11:. PubMed
  9. Isakova-Sivak I, et al. 2020. Vaccines (Basel). 8:00. PubMed
  10. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  11. Perry JSA, et al. 2018. Immunity. 48:923. PubMed
  12. Pfenninger P, et al. 2022. Front Immunol. 13:777113. PubMed
  13. Mamedov MR, et al. 2018. Immunity. 48:350. PubMed
  14. Lee CG, et al. 2019. JCI Insight. 4:5. PubMed
  15. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  16. Kemp V, et al. 2018. Cancer Gene Ther. 26:268. PubMed
  17. Goh PK, et al. 2022. Sci Adv. 8:eabk3338. PubMed
  18. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  19. Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
  20. Yue X, et al. 2021. EMBO Rep. 22:e52716. PubMed
  21. Zhang J, et al. 2022. Biomater Res. 26:44. PubMed
  22. Lu X, et al. 2019. Circ Res. 125:1055. PubMed
  23. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  24. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  25. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  26. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  27. Boulch M, et al. 2021. Sci Immunol. 6:. PubMed
  28. Culina S, et al. 2015. Diabetes. 64: 3532 - 3542. PubMed
  29. Kong IY, et al. 2020. Cell Rep. 33:108290. PubMed
RRID
AB_10900082 (BioLegend Cat. No. 104435) AB_2562560 (BioLegend Cat. No. 104436)

Antigen Details

Structure
Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Distribution

Subsets of B and T cells, monocytes, granulocytes, subset of NK cells

Function
Lymphocyte homing to HEV, rolling on activated endothelium
Ligand/Receptor
CD34, GlyCAM-1, MAdCAM-1
Cell Type
B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.

Gene ID
20343 View all products for this Gene ID
UniProt
View information about CD62L on UniProt.org

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