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首页 > 抗体蛋白 > 抗体
APC/Cyanine7 anti-mouse CD62L Antibody
产品名称：
APC/Cyanine7 anti-mouse CD62L Antibody
产品类别：
抗体
产品编号：
104427
产品应用：
104427
[价格]
规格 价格 库存
25µg ￥ 1128 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/eb mouse B lymphoma 38C-13
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4, in vivo and in vitro blocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 104457-104462).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
  2. Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
  3. Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
  4. Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
  5. Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
  6. Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
  7. Olver S, et al. 2006. Cancer Res. 66:571.
  8. Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
  9. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Lee JW, et al. 2006. Nature Immunol. 8:181.
  12. Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
  13. de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed
Product Citations
  1. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  2. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  3. Kurosawa M, et al. 2021. Int J Mol Sci. 22:. PubMed
  4. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 5:64. PubMed
  5. Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed
  6. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  7. Lin R, et al. 2020. Sci Rep. 10:14397. PubMed
  8. Volpedo G, et al. 2022. NPJ Vaccines. 7:32. PubMed
  9. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  10. Haque M, et al. 2021. STAR Protoc. 2:100264. PubMed
  11. Xu F, et al. 2022. Cell Death Discov. 8:142. PubMed
  12. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  13. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
  14. Zhang F, et al. 2019. Nat Commun. 10:3974. PubMed
  15. Fumagalli V, et al. 2020. J Exp Med. :217. PubMed
  16. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  17. Topham T 2010. J Immunol. 184:3841. PubMed
  18. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  19. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  20. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  21. Vieyra-Garcia P, et al. 2016. Clin Cancer Res. 22: 3328 - 3339. PubMed
  22. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  23. Ly A, et al. 2020. Cell Reports. 29(8):2257-2269.e6.. PubMed
  24. Rengarajan S, et al. 2020. Cell Rep Med. :1. PubMed
  25. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  26. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  27. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  28. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  29. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  30. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  31. Webb ER, et al. 2022. iScience. 25:104995. PubMed
  32. Studniberg SI, et al. 2022. Mol Syst Biol. 18:e10824. PubMed
  33. Toomer K, et al. 2016. J Immunol. 196: 3665 - 3676. PubMed
  34. Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
  35. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  36. Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed
  37. Pardo E, et al. 2017. PLoS One. 12(6):e0177472. PubMed
  38. Kim MY, et al. 2022. Nat Commun. 13:3296. PubMed
  39. Frantz PN, et al. 2021. Nat Commun. 12:6277. PubMed
  40. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  41. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  42. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 0.252777778. PubMed
  43. Ryg-Cornejo V, et al. 2016. Cell Rep. 14:68-81. PubMed
  44. Hebbandi Nanjundappa R, et al. 2017. Cell. 171:655. PubMed
  45. Olguín J, et al. 2015. Microbes Infect. 17: 586-595. PubMed
RRID
AB_830798 (BioLegend Cat. No. 104427) AB_830799 (BioLegend Cat. No. 104428)

Antigen Details

Structure
Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Distribution

Subsets of B and T cells, monocytes, granulocytes, subset of NK cells

Function
Lymphocyte homing to HEV, rolling on activated endothelium
Ligand/Receptor
CD34, GlyCAM-1, MAdCAM-1
Cell Type
B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.

Gene ID
20343 View all products for this Gene ID
UniProt
View information about CD62L on UniProt.org

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