Alexa Fluor® 647 anti-mouse CD62L Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1212	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

C3H/eb mouse B lymphoma 38C-13

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for other applications.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1-3, complement-dependent cytotoxicity⁴, in vivo and in vitro blocking of adhesion^1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections⁶. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 104457-104462).

Application References (PubMed link indicates BioLegend citation)

Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
Olver S, et al. 2006. Cancer Res. 66:571.
Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
Lee JW, et al. 2006. Nature Immunol. 8:181.
Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed

Product Citations

Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
del Rio ML, et al. 2021. Transl Res. Online ahead of print.. PubMed
Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
Wang Z et al. 2018. Immunity. 49(1):80-92 . PubMed
Meininger I, et al. 2016. Nat Commun. 7: 11292. PubMed

RRID

AB_493379 (BioLegend Cat. No. 104421)

Antigen Details

Structure: Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Distribution: Subsets of B and T cells, monocytes, granulocytes, subset of NK cells
Function: Lymphocyte homing to HEV, rolling on activated endothelium
Ligand/Receptor: CD34, GlyCAM-1, MAdCAM-1
Cell Type: B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.
Gene ID: 20343 View all products for this Gene ID
UniProt: View information about CD62L on UniProt.org

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