Purified anti-mouse/human PNAd Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 852	1

Product Details

Verified Reactivity

Mouse, Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse lymph node stromal cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

IHC-F - Quality tested
IHC-P - Verified
FC, IP, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5?- 10 ?g/ml is suggested.?For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 ?g/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking^1-4 of cell adhesion, immunohistochemical staining^1,2,6 of acetone-fixed frozen sections and 4% paraformaldehyde-fixed paraffin-embedded sections, flow cytometry^10,11,?immunoprecipitation^3,5, and Western blotting^6,11.

Application References (PubMed link indicates BioLegend citation)

Streeter PR, et al. 1988. J. Cell Biol. 107:1853. (IHC, Block)
Michie SA, et al. 1993. Amer. J. Path. 143:1688. (IHC, Block)
Berg EL, et al. 1991. J. Cell Biol. 114:343. (IP, Block)
Berg EL, et al. 1993. Nature 366:695. (Block)
Hemmerich S, et al. 1994. J. Exp. Med. 180:2219. (IP)
Samulowitz U, et al. 2002. Am. J. Path. 160:1669. (IHC, WB)
Burke SD, et al. 2007. Diabetes 56:2919. PubMed
Hirakawa J, et al. 2010. J. Biol. Chem. 285:40864. PubMed
Thomas SN, et al. 2012. J. Immunol. 189:2181. PubMed.
Izawa D, et al. 1999. Int. Immunol. 11(12):1989-98. (FC, ICC)
Sinha RK, et al. 2006. Immunology. 119(4):461-9. (FC, IHC-F, WB)

Product Citations

Hirakawa J, et al. 2010. J Biol Chem. 285:40864. PubMed
Nagy D, et al. 2021. Commun Biol. 4:993. PubMed
Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
Thomas S, et al. 2012. J Immunol. 189:2181. PubMed
Menzel L, et al. 2022. STAR Protoc. 3:101267. PubMed
Li Q, et al. 2020. Int J Oral Sci. 12:24. PubMed
Vito A, et al. 2021. Commun Biol. 4:859. PubMed
Noort A, et al. 2015. Am J Pathol. 185: 1935-1943. PubMed
Burke S, et al. 2007. Diabetes. 56:2919. PubMed
Jiao S, et al. 2022. J Immunother Cancer. 10:. PubMed
Lynskey N, et al. 2015. PLoS One. 11: 1005137. PubMed

RRID

AB_493554 (BioLegend Cat. No. 120801) AB_493555 (BioLegend Cat. No. 120802)

Antigen Details

Structure: Sulphated CD34, GlyCAM-1 and MAdCAM-1, Sgp200, sialomucin
Distribution: High endothelial venules (HEV) in lymphoid tissues, inflamed tissues and rheumatoid synovia
Function: Leukocyte homing, rolling
Ligand/Receptor: CD62L
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules
Antigen References: 1. Streeter PR, et al. 1988. J. Cell Biol. 107:1853.
2. Berg EL, et al. 1993. Nature 366:695.
3. Hemmerich S, et al. 1994. J. Exp. Med. 180:2219.
4. Mitoma J, et al. 2003. J. Biol. Chem. 278:9953.
5. Bruehl RE, et al. 2000. J. Biol. Chem. 275:32642.
6. Bistrup A, et al. 2004. Am. J. Pathol. 164:1635
Gene ID: 123803 View all products for this Gene ID 18203 View all products for this Gene ID
UniProt: View information about PNAd on UniProt.org

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