Biotin anti-mouse/human PNAd Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 996	1

Product Details

Verified Reactivity

Mouse, Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse lymph node stromal cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

IHC-F?- Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections.?For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 ?g per mL. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 ?g per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking^1-4 of cell adhesion, immunohistochemical staining^1,2,6 of acetone-fixed frozen sections and 4% paraformaldehyde-fixed paraffin-embedded sections, flow cytometry^10,11,?immunoprecipitation^3,5, and Western blotting^6,11.

Application References (PubMed link indicates BioLegend citation)

Streeter PR, et al. 1988. J. Cell Biol. 107:1853. (IHC, Block)
Michie SA, et al. 1993. Amer. J. Path. 143:1688. (IHC, Block)
Berg EL, et al. 1991. J. Cell Biol. 114:343. (IP, Block)
Berg EL, et al. 1993. Nature 366:695. (Block)
Hemmerich S, et al. 1994. J. Exp. Med. 180:2219. (IP)
Samulowitz U, et al. 2002. Am. J. Path. 160:1669. (IHC, WB)
Burke SD, et al. 2007. Diabetes 56:2919. PubMed
Hirakawa J, et al. 2010. J. Biol. Chem. 285:40864. PubMed
Thomas SN, et al. 2012. J. Immunol. 189:2181. PubMed.
Izawa D, et al. 1999. Int. Immunol. 11(12):1989-98. (FC, ICC)
Sinha RK, et al. 2006. Immunology. 119(4):461-9. (FC, IHC-F, WB)

Product Citations

Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
Folgosa L, et al. 2013. J Immunol. 191:5951. PubMed
van Hooren L, et al. 2021. Nature Communications. 12(1):4127. PubMed
He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
Onder L et al. 2017. Immunity. 47(1):80-92 . PubMed
Xiong H, et al. 2021. Theranostics. 11:1594. PubMed
Bombardieri M, et al. 2012. J Immunol. 189:3767. PubMed
He W, et al. 2020. Frontiers in Oncology. 1.097222222. PubMed
Ligon MM, et al. 2020. Mucosal Immunol. 1.172222222. PubMed
Rodriguez AB, et al. 2022. Front Immunol. 13:1009306. PubMed
Tsuboi K, et al. 2013. J Immunol. 191:448. PubMed
Yan X, et al. 2015. PLoS One. 10: 0145218. PubMed
Huppé CA, et al. 2018. Mucosal Immunol. 0.536111111. PubMed

RRID

AB_493556 (BioLegend Cat. No. 120803) AB_493557 (BioLegend Cat. No. 120804)

Antigen Details

Structure: Sulphated CD34, GlyCAM-1 and MAdCAM-1, Sgp200, sialomucin
Distribution: High endothelial venules (HEV) in lymphoid tissues, inflamed tissues and rheumatoid synovia
Function: Leukocyte homing, rolling
Ligand/Receptor: CD62L
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules
Antigen References: 1. Streeter PR, et al. 1988. J. Cell Biol. 107:1853.
2. Berg EL, et al. 1993. Nature 366:695.
3. Hemmerich S, et al. 1994. J. Exp. Med. 180:2219.
4. Mitoma J, et al. 2003. J. Biol. Chem. 278:9953.
5. Bruehl RE, et al. 2000. J. Biol. Chem. 275:32642.
6. Bistrup A, et al. 2004. Am. J. Pathol. 164:1635
Gene ID: 123803 View all products for this Gene ID 18203 View all products for this Gene ID
UniProt: View information about PNAd on UniProt.org

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