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Alexa Fluor® 647 anti-mouse/human PNAd Antibod
产品名称：
Alexa Fluor® 647 anti-mouse/human PNAd Antibod
产品类别：
抗体
产品编号：
120807
产品应用：
120807
[价格]
规格 价格 库存
25µg ￥ 2052 1

产品详情

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse lymph node stromal cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking1-4 of cell adhesion, immunohistochemical staining1,2,6 of acetone-fixed frozen sections and 4% paraformaldehyde-fixed paraffin-embedded sections, flow cytometry10,11,?immunoprecipitation3,5, and Western blotting6,11.

Application References

(PubMed link indicates BioLegend citation)
  1. Streeter PR, et al. 1988. J. Cell Biol. 107:1853. (IHC, Block)
  2. Michie SA, et al. 1993. Amer. J. Path. 143:1688. (IHC, Block)
  3. Berg EL, et al. 1991. J. Cell Biol. 114:343. (IP, Block)
  4. Berg EL, et al. 1993. Nature 366:695. (Block)
  5. Hemmerich S, et al. 1994. J. Exp. Med. 180:2219. (IP)
  6. Samulowitz U, et al. 2002. Am. J. Path. 160:1669. (IHC, WB)
  7. Burke SD, et al. 2007. Diabetes 56:2919. PubMed
  8. Hirakawa J, et al. 2010. J. Biol. Chem. 285:40864. PubMed
  9. Thomas SN, et al. 2012. J. Immunol. 189:2181. PubMed.
  10. Izawa D, et al. 1999. Int. Immunol. 11(12):1989-98. (FC, ICC)
  11. Sinha RK, et al. 2006. Immunology. 119(4):461-9. (FC, IHC-F, WB)
Product Citations
  1. Matryba P, et al. 2020. J Immunol. 1395:204. PubMed
RRID
AB_2783059 (BioLegend Cat. No. 120807) AB_2783060 (BioLegend Cat. No. 120808)

Antigen Details

Structure
Sulphated CD34, GlyCAM-1 and MAdCAM-1, Sgp200, sialomucin
Distribution

High endothelial venules (HEV) in lymphoid tissues, inflamed tissues and rheumatoid synovia

Function
Leukocyte homing, rolling
Ligand/Receptor
CD62L
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules
Antigen References

1. Streeter PR, et al. 1988. J. Cell Biol. 107:1853.
2. Berg EL, et al. 1993. Nature 366:695.
3. Hemmerich S, et al. 1994. J. Exp. Med. 180:2219.
4. Mitoma J, et al. 2003. J. Biol. Chem. 278:9953.
5. Bruehl RE, et al. 2000. J. Biol. Chem. 275:32642.
6. Bistrup A, et al. 2004. Am. J. Pathol. 164:1635

Gene ID
123803 View all products for this Gene ID 18203 View all products for this Gene ID
UniProt
View information about PNAd on UniProt.org

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