Ultra-LEAF™ Purified anti-mouse MAdCAM-1 Ant-上海雅吉生物科技有限公司

规格	价格	库存
100mg	￥ 40596	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Endothelial cells

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of lymphocyte adhesion and in vivo blocking of lymphocyte homing^1-4,7, immunohistochemical staining^1,5,6 of acetone-fixed frozen sections, immunoprecipitation, and Western blotting¹.

Application References (PubMed link indicates BioLegend citation)

Streeter PR, et al. 1988. Nature 331:41.
Briskin MJ, et al. 1993. Nature 363:461.
Berlin C, et al. 1993. Cell 74:185.
Bargatze RF, et al. 1995. Immunity 3:99.
Tanneau GM, et al. 1999. J. Histochem. Cytochem. 47:1581.
Savinov AY, et al. 2003. J. Exp. Med. 197:643.
Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343.
Hindley JP, et al. 2012. Cancer Res. 72:5473. PubMed.

RRID

AB_2832426 (BioLegend Cat. No. 120715) AB_2832427 (BioLegend Cat. No. 120716) AB_2832428 (BioLegend Cat. No. 120717) AB_2876436 (BioLegend Cat. No. 120718) AB_2876436 (BioLegend Cat. No. 120719) AB_2876436 (BioLegend Cat. No. 120720)

Antigen Details

Structure: Type I glycoprotein, Ig superfamily
Distribution: HEV of mucosal lymphoid organ and lamina propia, endothelial cells in mammary glands and pancreas
Function: Lymphocyte tethering, rolling and homing, costimulate T cell proliferatioon
Ligand/Receptor: Integrin a4?7, CD62L
Cell Type: Endothelial cells
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules
Antigen References: 1. Streeter PR, et al. 1988. Nature 331:41
2. Briskin MJ, et al. 1993.Nature 363:461.
3. Berlin C, et al. 1993. Cell 74:185.
4. Lehnert K, et al. 1998. Eur. J. Immunol. 28:3605.
5. Picarella D, et al. 1997. J. Immunol. 158:2099.
Gene ID: 17123 View all products for this Gene ID
UniProt: View information about MAdCAM-1 on UniProt.org

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