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首页 > 抗体蛋白 > 抗体
Biotin anti-mouse MAdCAM-1 Antibody
产品名称：
Biotin anti-mouse MAdCAM-1 Antibody
产品类别：
抗体
产品编号：
120705
产品应用：
120705
[价格]
规格 价格 库存
50µg ￥ 996 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Endothelial cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of lymphocyte adhesion and in vivo blocking of lymphocyte homing1-4,7, immunohistochemical staining1,5,6 of acetone-fixed frozen sections, immunoprecipitation, and Western blotting1.

Application References

(PubMed link indicates BioLegend citation)
  1. Streeter PR, et al. 1988. Nature 331:41.
  2. Briskin MJ, et al. 1993. Nature 363:461.
  3. Berlin C, et al. 1993. Cell 74:185.
  4. Bargatze RF, et al. 1995. Immunity 3:99.
  5. Tanneau GM, et al. 1999. J. Histochem. Cytochem. 47:1581.
  6. Savinov AY, et al. 2003. J. Exp. Med. 197:643.
  7. Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343.
  8. Hindley JP, et al. 2012. Cancer Res. 72:5473. PubMed.
Product Citations
  1. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  2. Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
  3. Prados A, et al. 2021. Nat Immunol. 22:510. PubMed
  4. Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
  5. Xiong H, et al. 2021. Theranostics. 11:1594. PubMed
  6. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  7. Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed
  8. Song W, et al. 2022. Immunity. 55:290. PubMed
RRID
AB_493396 (BioLegend Cat. No. 120705) AB_493397 (BioLegend Cat. No. 120706)

Antigen Details

Structure
Type I glycoprotein, Ig superfamily
Distribution

HEV of mucosal lymphoid organ and lamina propia, endothelial cells in mammary glands and pancreas

Function
Lymphocyte tethering, rolling and homing, costimulate T cell proliferatioon
Ligand/Receptor
Integrin a4?7, CD62L
Cell Type
Endothelial cells
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules
Antigen References

1. Streeter PR, et al. 1988. Nature 331:41
2. Briskin MJ, et al. 1993.Nature 363:461.
3. Berlin C, et al. 1993. Cell 74:185.
4. Lehnert K, et al. 1998. Eur. J. Immunol. 28:3605.
5. Picarella D, et al. 1997. J. Immunol. 158:2099.

Gene ID
17123 View all products for this Gene ID
UniProt
View information about MAdCAM-1 on UniProt.org

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