Alexa Fluor® 488 anti-mouse MAdCAM-1 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1212	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Endothelial cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of lymphocyte adhesion and in vivo blocking of lymphocyte homing^1-4,7, immunohistochemical staining^1,5,6 of acetone-fixed frozen sections, immunoprecipitation, and Western blotting¹.

Application References (PubMed link indicates BioLegend citation)

Streeter PR, et al. 1988. Nature 331:41.
Briskin MJ, et al. 1993. Nature 363:461.
Berlin C, et al. 1993. Cell 74:185.
Bargatze RF, et al. 1995. Immunity 3:99.
Tanneau GM, et al. 1999. J. Histochem. Cytochem. 47:1581.
Savinov AY, et al. 2003. J. Exp. Med. 197:643.
Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343.
Hindley JP, et al. 2012. Cancer Res. 72:5473. PubMed.

Product Citations

Morrison B, et al. 2012. PLoS One. 7:e52692. PubMed
Kato T, et al. 2019. Front Immunol. 10:2021. PubMed
Kunimura K, et al. 2020. Cell Reports. 29(9):2823-2834.e7.. PubMed
Kometani K, et al. 2011. J Exp Med. 208:1447. PubMed
Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed

RRID

AB_493399 (BioLegend Cat. No. 120707) AB_493398 (BioLegend Cat. No. 120708)

Antigen Details

Structure: Type I glycoprotein, Ig superfamily
Distribution: HEV of mucosal lymphoid organ and lamina propia, endothelial cells in mammary glands and pancreas
Function: Lymphocyte tethering, rolling and homing, costimulate T cell proliferatioon
Ligand/Receptor: Integrin a4?7, CD62L
Cell Type: Endothelial cells
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules
Antigen References: 1. Streeter PR, et al. 1988. Nature 331:41
2. Briskin MJ, et al. 1993.Nature 363:461.
3. Berlin C, et al. 1993. Cell 74:185.
4. Lehnert K, et al. 1998. Eur. J. Immunol. 28:3605.
5. Picarella D, et al. 1997. J. Immunol. 158:2099.
Gene ID: 17123 View all products for this Gene ID
UniProt: View information about MAdCAM-1 on UniProt.org

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