Biotin anti-mouse Panendothelial Cell Antigen Anti
- 产品名称:
- Biotin anti-mouse Panendothelial Cell Antigen Anti
- 产品类别:
- 抗体
- 产品编号:
- 120503
- 产品应用:
- 120503
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse lymph node stromal cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (in the relevant formats) include: immunoprecipitation, Western blotting, immunocytochemistry, and immunohistochemical staining of acetone-fixed frozen sections2-5. Clone MECA-32 is not recommended for formalin-fixed paraffin sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Leppink DM, et al. 1989. Transplantation 48:874.
- Bergese SD, et al. 1994. Transplantation 57:711. (IHC)
- Tanneau GM, et al. 1999. J. Histochem. Cytochem. 47:1581. (IHC)
- Kopp HG, et al. 2005. Blood 106:505. (IHC)
- Hallmann R, et al. 1995. Dev. Dyn. 202:325. (IHC)
- Product Citations
-
- Nakagawa T, et al. 2021. Cell Rep. 37:109875. PubMed
- Horwitz E, et al. 2014. Cancer Discovery. 4:730. PubMed
- RRID
- AB_493303 (BioLegend Cat. No. 120503) AB_493304 (BioLegend Cat. No. 120504)
- Structure
- 50-55 kD homodimer
- Distribution
-
Endothelial cell in the embryonic and the adult mice, except in the brain and in skeletal and cardiac muscle, where it has more restricted distribution
- Cell Type
- Embryonic Stem Cells, Endothelial cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules
- Antigen References
-
1. Leppnik DM, et al. 1989. Transplantation 48:874.
2. Hallmann R, et al. 1995. Dev. Dyn. 202:325.
3. Kruse A, et al. 1999. Bio. Reprod. 61:1393.
- Gene ID
- 84094 View all products for this Gene ID
- UniProt
- View information about Panendothelial Cell Antigen on UniProt.org