Alexa Fluor® 488 anti-mouse Panendothelial Cel
- 产品名称：
- Alexa Fluor® 488 anti-mouse Panendothelial Cel
- 产品类别：
- 抗体
- 产品编号：
- 120505
- 产品应用：
- 120505
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse lymph node stromal cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (in the relevant formats) include: immunoprecipitation, Western blotting, immunocytochemistry, and immunohistochemical staining of acetone-fixed frozen sections2-5. Clone MECA-32 is not recommended for formalin-fixed paraffin sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Leppink DM, et al. 1989. Transplantation 48:874.
- Bergese SD, et al. 1994. Transplantation 57:711. (IHC)
- Tanneau GM, et al. 1999. J. Histochem. Cytochem. 47:1581. (IHC)
- Kopp HG, et al. 2005. Blood 106:505. (IHC)
- Hallmann R, et al. 1995. Dev. Dyn. 202:325. (IHC)
- Product Citations
-
- Auvinen K, et al. 2019. Sci Rep. 9:15698. PubMed
- Liu Y, et al. 2020. Cell. 183(6):1665-1681.e18. PubMed
- RRID
- AB_493332 (BioLegend Cat. No. 120505) AB_493305 (BioLegend Cat. No. 120506)
- Structure
- 50-55 kD homodimer
- Distribution
-
Endothelial cell in the embryonic and the adult mice, except in the brain and in skeletal and cardiac muscle, where it has more restricted distribution
- Cell Type
- Embryonic Stem Cells, Endothelial cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules
- Antigen References
-
1. Leppnik DM, et al. 1989. Transplantation 48:874.
2. Hallmann R, et al. 1995. Dev. Dyn. 202:325.
3. Kruse A, et al. 1999. Bio. Reprod. 61:1393.
- Gene ID
- 84094 View all products for this Gene ID
- UniProt
- View information about Panendothelial Cell Antigen on UniProt.org
