PE/Dazzle™ 594 anti-mouse CD34 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 2052	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Cells transfected with mouse CD34

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Dazzle? 594 under optimal conditions.

Concentration

0.2 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle? 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

The MEC14.7 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting⁶, and immunohistochemistry of acetone-fixed frozen sections and paraffin-embedded sections^2,4,5,6.

Application References (PubMed link indicates BioLegend citation)

Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368. (FC)
Knowles HJ, et al. 2004. Circ. Res. 95:162. (IHC)
Trempus CS, et al. 2003. J. Invest. Dermatol. 120:501.
Winding B, et al. 2002. Clin. Cancer Res. 8:1932. (IHC)
Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
Kairaitis LK, et al. 2005. Am. J. Physiol. Renal. Physiol. 288:F198. (IHC, WB)
Ao A, et al. 2008. P. Natl. Acad. Sci. USA 105:7821. PubMed
Zaynagetdinov R., et al. 2011. J Immunol. 187:5703. PubMed.

RRID

AB_2832422 (BioLegend Cat. No. 119329) AB_2876434 (BioLegend Cat. No. 119330)

Antigen Details

Structure: Type I membrane protein, 75-120 kD, highly glycosylated; two isoforms reported
Distribution: Hematopoietic progenitors, brain, testis, endothelial cells; low expression in thymus, spleen, and bone marrow
Function: Possible adhesion molecule thought to function in early hematopoiesis by mediating attachment of stem cells to bone marrow extracellular matrix or stromal cells. Presents carbohydrate ligands to selectins.
Ligand/Receptor: L-selectin, other selectins
Cell Type: Endothelial cells, Hematopoietic stem and progenitors
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368.
2. Brown J, et al. 1991. Int. Immunol. 3:175.
3. Suda J, et al. 1992. Blood 79:2288.
4. Baumhueter S, et al. 1994. Blood 84:2554.
Gene ID: 12490 View all products for this Gene ID
UniProt: View information about CD34 on UniProt.org

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