首页 > 抗体蛋白 > 抗体
PE anti-mouse CD34 Antibody
产品名称:
PE anti-mouse CD34 Antibody
产品类别:
抗体
产品编号:
119307
产品应用:
119307
[价格]
规格 价格 库存
50µg ¥ 1320 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Cells transfected with mouse CD34
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The MEC14.7 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting6, and immunohistochemistry of acetone-fixed frozen sections and paraffin-embedded sections2,4,5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368. (FC)
  2. Knowles HJ, et al. 2004. Circ. Res. 95:162. (IHC)
  3. Trempus CS, et al. 2003. J. Invest. Dermatol. 120:501.
  4. Winding B, et al. 2002. Clin. Cancer Res. 8:1932. (IHC)
  5. Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
  6. Kairaitis LK, et al. 2005. Am. J. Physiol. Renal. Physiol. 288:F198. (IHC, WB)
  7. Ao A, et al. 2008. P. Natl. Acad. Sci. USA 105:7821. PubMed
  8. Zaynagetdinov R., et al. 2011. J Immunol. 187:5703. PubMed.
Product Citations
  1. Chen X, et al. 2022. Front Bioeng Biotechnol. 10:915181. PubMed
  2. Guo L, et al. 2019. Mol Cell. 73:815. PubMed
  3. Mardomi A, et al. 2021. J Cell Physiol. 236:6328. PubMed
  4. Paris AJ, et al. 2020. Nat Cell Biol. 1.747916667. PubMed
  5. Cha S, et al. 2021. Int J Mol Sci. 22:. PubMed
  6. Jiang M, et al. 2020. Exp Ther Med. 1.989583333. PubMed
  7. Zhang L, et al. 2022. Front Genet. 13:847646. PubMed
  8. Amouzegar A, et al. 2017. Stem Cells. 10.1002/stem.2611. PubMed
  9. Zhang S, et al. 2021. Stem Cells Int. 6616240:2021. PubMed
  10. Duan Y, et al. 2018. Stem Cells. 36:1430. PubMed
  11. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  12. Wei Q, et al. 2020. Dev Cell. 53:503. PubMed
  13. Dai Z, et al. 2021. J Control Release. 336:433. PubMed
  14. Bao Z, et al. 2020. Ann Transplant. 25:e921287. PubMed
  15. Yang X, et al. 2021. Bioact Mater. 3150:6. PubMed
  16. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  17. Zhu Q, et al. 2021. J Am Heart Assoc. 10:e023491. PubMed
RRID
AB_493401 (BioLegend Cat. No. 119307) AB_493400 (BioLegend Cat. No. 119308)

Antigen Details

Structure
Type I membrane protein, 75-120 kD, highly glycosylated; two isoforms reported
Distribution

Hematopoietic progenitors, brain, testis, endothelial cells; low expression in thymus, spleen, and bone marrow

Function
Possible adhesion molecule thought to function in early hematopoiesis by mediating attachment of stem cells to bone marrow extracellular matrix or stromal cells. Presents carbohydrate ligands to selectins.
Ligand/Receptor
L-selectin, other selectins
Cell Type
Endothelial cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368.
2. Brown J, et al. 1991. Int. Immunol. 3:175.
3. Suda J, et al. 1992. Blood 79:2288.
4. Baumhueter S, et al. 1994. Blood 84:2554.

Gene ID
12490 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org

联系我们

TEL:021-34661275  点击拨打热线