Brilliant Violet 421™ anti-mouse CD34 Antibo-上海雅吉生物科技有限公司

规格	价格	库存
125µL	￥ 2448	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Cells transfected with mouse CD34

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

The MEC14.7 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting⁶, and immunohistochemistry of acetone-fixed frozen sections and paraffin-embedded sections^2,4,5,6.

Application References (PubMed link indicates BioLegend citation)

Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368. (FC)
Knowles HJ, et al. 2004. Circ. Res. 95:162. (IHC)
Trempus CS, et al. 2003. J. Invest. Dermatol. 120:501.
Winding B, et al. 2002. Clin. Cancer Res. 8:1932. (IHC)
Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
Kairaitis LK, et al. 2005. Am. J. Physiol. Renal. Physiol. 288:F198. (IHC, WB)
Ao A, et al. 2008. P. Natl. Acad. Sci. USA 105:7821. PubMed
Zaynagetdinov R., et al. 2011. J Immunol. 187:5703. PubMed.

Product Citations

Shao Y, et al. 2019. Diabetes. 68:2131. PubMed
Papafragkos I, et al. 2022. Front Immunol. 13:889075. PubMed
Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
Shook BA, et al. 2020. Cell Stem Cell. 26(6):880-895. PubMed
Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
Sebo ZL, et al. 2021. Mol Metab. 44:101141. PubMed
Sebo ZL, et al. 2018. Development. 145. PubMed
Shao Y, et al. 2019. Stem Cells. 37:1331. PubMed

RRID

AB_10900980 (BioLegend Cat. No. 119321)

Antigen Details

Structure: Type I membrane protein, 75-120 kD, highly glycosylated; two isoforms reported
Distribution: Hematopoietic progenitors, brain, testis, endothelial cells; low expression in thymus, spleen, and bone marrow
Function: Possible adhesion molecule thought to function in early hematopoiesis by mediating attachment of stem cells to bone marrow extracellular matrix or stromal cells. Presents carbohydrate ligands to selectins.
Ligand/Receptor: L-selectin, other selectins
Cell Type: Endothelial cells, Hematopoietic stem and progenitors
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368.
2. Brown J, et al. 1991. Int. Immunol. 3:175.
3. Suda J, et al. 1992. Blood 79:2288.
4. Baumhueter S, et al. 1994. Blood 84:2554.
Gene ID: 12490 View all products for this Gene ID
UniProt: View information about CD34 on UniProt.org

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