Ultra-LEAF™ Purified anti-mouse CD31 Antibod-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2772	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Polyoma middle T transformed EC line tEnd.1

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IP, Block, IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.⁸ Additional reported applications (in the relevant formats) include: immunoprecipitation¹, in vitro and in vivo blocking of CD31-mediated cell-cell interactions^1-4, immunohistochemical staining^1,5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)^12,13.
Special Note: The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 102529 and 102530).

Application References (PubMed link indicates BioLegend citation)

Vecchi A, et al. 1994. Eur. J. Cell Biol. 63:247. (IP, IHC, Block)
Christofidou-Solomidou M, et al. 1997. J. Immunol. 158:4872. (Block)
DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
Rosenblum WI, et al. 1994. Am. J. Pathol. 145:33. (Block)
Baldwin HS, et al. 1994. Development 120:2539. (IHC)
Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
Leung VW, et al. 2009. Am J. Pathol. 175:1757. PubMed
Chacko AM, et al. 2012. PLoS One 7:e34958.
Giacomini C, et al. 2014. Exp Eye Res. 18:1. PubMed
Morita R, et al. 2015. PNAS. 112:160. PubMed
Ito A, et al. 2015. Brain Res. 1594:310. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Inverso D, et al. 2021. Developmental Cell. 56(11):1677-1693.e10. PubMed

RRID

AB_2832292 (BioLegend Cat. No. 102529) AB_2832293 (BioLegend Cat. No. 102530)

Antigen Details

Structure: Ig superfamily, 130-140 kD
Distribution: Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets
Function: Adhesion
Ligand/Receptor: CD38, α_V/β₃ integrin
Cell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.
Gene ID: 18613 View all products for this Gene ID
UniProt: View information about CD31 on UniProt.org

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