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首页 > 抗体蛋白 > 抗体
Purified anti-mouse CD31 Antibody
产品名称：
Purified anti-mouse CD31 Antibody
产品类别：
抗体
产品编号：
102501
产品应用：
102501
[价格]
规格 价格 库存
50µg ￥ 852 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Polyoma middle T transformed EC line tEnd.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, Block, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (in the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, immunohistochemical staining1,5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)12,13.
Special Note: The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 102529 and 102530).

Application References

(PubMed link indicates BioLegend citation)
  1. Vecchi A, et al. 1994. Eur. J. Cell Biol. 63:247. (IP, IHC, Block)
  2. Christofidou-Solomidou M, et al. 1997. J. Immunol. 158:4872. (Block)
  3. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  4. Rosenblum WI, et al. 1994. Am. J. Pathol. 145:33. (Block)
  5. Baldwin HS, et al. 1994. Development 120:2539. (IHC)
  6. Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
  7. Leung VW, et al. 2009. Am J. Pathol. 175:1757. PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Giacomini C, et al. 2014. Exp Eye Res. 18:1. PubMed
  10. Morita R, et al. 2015. PNAS. 112:160. PubMed
  11. Ito A, et al. 2015. Brain Res. 1594:310. PubMed
  12. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  13. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Betterman KL, et al. 2020. J Clin Invest. 130:3315. PubMed
  3. Brenner JS, et al. 2018. Nat Commun. 9:20222. PubMed
  4. Todd L, et al. 2019. J Neuroinflammation. 16:118. PubMed
  5. Shafer MER, et al. 2017. Stem Cell Reports. 1.040277778. PubMed
  6. Lei L, et al. 2020. Stem Cell Res Ther. 11:137. PubMed
  7. Schnoor M, et al. 2011. J Exp Med. 208:1721. PubMed
  8. Reglero-Real N, et al. 2021. Immunity. :. PubMed
  9. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  10. Liu X et al. 2019. Immunity. 50(2):317-333 . PubMed
  11. Challa DK, et al. 2019. MAbs. 11:848. PubMed
  12. Marchant C, et al. 2020. Development. 147:00:00. PubMed
  13. Liu Y, et al. 2019. J Cell Physiol. 234:9525. PubMed
  14. Juan T, et al. 2009. Clin Cancer Res. 3.819444444. PubMed
  15. Dertschnig S, et al. 2020. J Clin Invest. 130:1896. PubMed
  16. Gao J, et al. 2017. Oncol Lett. 14:2954. PubMed
  17. Lobo P, et al. 2012. J Immunol. 188:1675. PubMed
  18. Dooley J, et al. 2020. Cell Rep. 32:107880. PubMed
  19. Yang X, et al. 2020. Cell Biosci. 0.488194444. PubMed
  20. Carr MJ, et al. 2019. Cell Stem Cell. 24:240. PubMed
  21. Song A, et al. 2018. Sci Rep. 8:723. PubMed
  22. O'Dea KP, et al. 2020. J Extracell Vesicles. 9:1706708. PubMed
  23. Baluk P, et al. 2020. Am J Pathol. 190:2355. PubMed
  24. Opzoomer JW, et al. 2021. Sci Adv. 7:eabg9518. PubMed
  25. Woyciechowski S, Hofmann M, Pircher H 2017. Eur J Immunol. 47:244-250. PubMed
  26. Na YR, et al. 2020. Cell Rep. 107643:31. PubMed
  27. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  28. Crosse EI, et al. 2020. Cell Stem Cell. 27:822. PubMed
  29. Fu H, et al. 2019. Front Immunol. 10:271. PubMed
  30. Sun W, et al. 2021. Mol Ther. 29:1312. PubMed
  31. Hansmeier NR, et al. 2022. Int J Mol Sci. 23:. PubMed
  32. Arumugam R, et al. 2020. Immunobiology. 151896:225. PubMed
  33. Shiuan E, et al. 2020. F1000Research. 0.525694444. PubMed
  34. Cardoso F, et al. 2021. Nature. 597:410. PubMed
  35. Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed
  36. Maul A, et al. 2022. Elife. 11:. PubMed
  37. Takahashi K, et al. 2020. J Biol Chem. 295:12559. PubMed
  38. Kuboi Y, et al. 2019. Int Immunol. 31:287. PubMed
  39. Poulos MG, et al. 2017. J Clin Invest. 127:4163. PubMed
  40. Ohnuki H, et al. 2012. Blood. 119:2688. PubMed
  41. Andreev D, et al. 2020. J Clin Invest. 130:4811. PubMed
  42. Matsumoto A, et al. 2021. iScience. 24:102839. PubMed
  43. Koch PS, et al. 2021. Front Physiol. 12:722394. PubMed
  44. Lee C, et al. 2020. J Shoulder Elbow Surg. 719:29. PubMed
  45. Ito A, et al. 2015. Brain Res. 1594:310. PubMed
  46. Sun JX, et al. 2018. Angiogenesis. 8:2407. PubMed
  47. Zlotoff D, et al. 2011. Blood. 118:1962. PubMed
  48. Heil J, et al. 2021. Nat Commun. 12:6963. PubMed
  49. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  50. Zakharova I, et al. 2022. Front Bioeng Biotechnol. 10:772981. PubMed
  51. Giacomini C, et al. 2014. Exp Eye Res. 18:1. PubMed
  52. Cheung K, et al. 2015. Proc Natl Acad Sci U S A. 112: E5815 - E5824. PubMed
  53. Genoud V, et al. 2018. Oncoimmunology. 7:e1501137. PubMed
  54. Storer MA, et al. 2020. Developmental Cell. 52(4):509-524.e9.. PubMed
  55. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  56. Barcia Durán JG, et al. 2021. Commun Biol. 4:406. PubMed
  57. Baluk P, et al. 2022. Methods Mol Biol. 2441:115. PubMed
  58. Kolb R, et al. 2016. Nat Commun. 7:13007. PubMed
  59. Fierle JK, et al. 2021. Cell Reports Medicine. 2(8):100362. PubMed
  60. Lee C, et al. 2020. J Orthop Res. 1159:38. PubMed
  61. Figueiredo CA, et al. 2022. J Neuroinflammation. 19:17. PubMed
  62. Morita R, et al. 2015. Proc Natl Acad Sci U S A. 112:160. PubMed
  63. Huynh H, et al. 2019. Int J Oncol. 54:1123. PubMed
  64. Kabir AU, et al. 2018. JCI Insight. 3. PubMed
RRID
AB_312908 (BioLegend Cat. No. 102501) AB_312909 (BioLegend Cat. No. 102502)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

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