PE anti-mouse CD59a Antibody
- 产品名称：
- PE anti-mouse CD59a Antibody
- 产品类别：
- 抗体
- 产品编号：
- 143103
- 产品应用：
- 143103
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- EL-4 cells transfected with murine CD59a
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume.? It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting of mouse erythrocytes1.
- Application References
(PubMed link indicates BioLegend citation) -
- Harris CL, et al. 2003. Immunology 109:117. (WB)
- Product Citations
-
- Janela B, et al. 2019. Immunity. 50:1069. PubMed
- Pais H, et al. 2019. Sci Rep. 9:5760. PubMed
- RRID
- AB_10965647 (BioLegend Cat. No. 143103) AB_10962439 (BioLegend Cat. No. 143104)
- Structure
- Glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein, 18-25 kD
- Distribution
- Different cell types, including endothelia, erythrocytes, and platelets
- Function
- Inhibits complement-mediated cell lysis by preventing the incorporation of factor C9 to the C5b-C8 complex, preventing the formation of the membrane attack complex (MAC)
- Interaction
- Complement factor C9
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Costimulatory Molecules
- Molecular Family
- CD Molecules
- Antigen References
-
1. Pu JJ and Brodsky RA. 2011. Clin. Transl. Sci. 4:219.
2. Yu Q, et al. 2010. Cell. Mol. Immunol. 7:334.
3. Kohli P and Levy BD. 2009. Br. J. Pharmacol. 158:960.
- Gene ID
- 12509 View all products for this Gene ID
- UniProt
- View information about CD59a on UniProt.org
