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Biotin anti-mouse CD30 Antibody
产品名称:
Biotin anti-mouse CD30 Antibody
产品类别:
抗体
产品编号:
102303
产品应用:
102303
[价格]
规格 价格 库存
50µg ¥ 924 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse CD30-mouse IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro induction of IL-5 production1. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 102306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 102304) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Bowen MA, et al. 1996. J. Immunol. 156:442.
  2. Punt JA, et al. 1997. J. Exp. Med. 186:1911.
Product Citations
  1. Ye Z, et al. 2010. Mol Immunol. 47:2411. PubMed
RRID
AB_2240925 (BioLegend Cat. No. 102303)

Antigen Details

Structure
TNFR superfamily, 105-120 kD
Distribution

Activated T cells, B cells and NK cells, monocytes

Function
Costimulation, regulates negative selection of thymocytes
Ligand/Receptor
CD153 (CD30L)
Cell Type
B cells, Embryonic Stem Cells, Monocytes, NK cells, T cells, Tregs
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Falini B, et al. 1995. Blood 85:1.
3. Gedrich RW, et al. 1996. J. Biol. Chem. 271:12852.

Gene ID
21941 View all products for this Gene ID
UniProt
View information about CD30 on UniProt.org

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