APC anti-mouse CD30 Antibody
- 产品名称:
- APC anti-mouse CD30 Antibody
- 产品类别:
- 抗体
- 产品编号:
- 102312
- 产品应用:
- 102312
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse CD30-mouse IgG1 fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: in vitro induction of IL-5 production1. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 102306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 102304) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).
- Application References
(PubMed link indicates BioLegend citation) -
- Bowen MA, et al. 1996. J. Immunol. 156:442.
- Punt JA, et al. 1997. J. Exp. Med. 186:1911.
- Product Citations
-
- Wang R, et al. 2017. Biomaterials. 10.1016/j.biomaterials.2017.03.016. PubMed
- RRID
- AB_2562412 (BioLegend Cat. No. 102312)
- Structure
- TNFR superfamily, 105-120 kD
- Distribution
-
Activated T cells, B cells and NK cells, monocytes
- Function
- Costimulation, regulates negative selection of thymocytes
- Ligand/Receptor
- CD153 (CD30L)
- Cell Type
- B cells, Embryonic Stem Cells, Monocytes, NK cells, T cells, Tregs
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Falini B, et al. 1995. Blood 85:1.
3. Gedrich RW, et al. 1996. J. Biol. Chem. 271:12852.
- Gene ID
- 21941 View all products for this Gene ID
- UniProt
- View information about CD30 on UniProt.org