Purified anti-human SSEA-4 Antibody
- 产品名称：
- Purified anti-human SSEA-4 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 330401
- 产品应用：
- 330401
- Verified Reactivity
- Human
- Reported Reactivity
- Mouse, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human embryonic carcinoma cell line 2102Ep
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide with stabilizer.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
ICC - Verified
IHC-P, IHC-F, ELISA - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: ELISA1 and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Kannagi R, et al. 1983. The EMBO J. 2:2355
- Product Citations
-
- van der Graaf LM, et al. 2020. Stem Cell Research. 39:101498.. PubMed
- Giri S, et al. 2020. Stem Cell Research. 39:101494.. PubMed
- Daoutsali E et al. 2019. Stem Cell Res. 1723:19:00 . PubMed
- Yang L, et al. 2018. Am J Physiol Lung Cell Mol Physiol. 9:1420. PubMed
- Meraviglia V, et al. 2020. Stem Cell Res. 101764:45. PubMed
- Schmid B, et al. 2021. Stem Cell Research. 52:102240. PubMed
- Yang L, et al. 2021. JCI Insight. 6:. PubMed
- Kim KP, et al. 2020. Stem Cell Research. 72.63819444. PubMed
- Themeli M, et al. 2020. Stem Cell Reports. 14:300. PubMed
- Xia H, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 320:L926. PubMed
- Gao X et al. 2018. Stem cell research. 31:193-196 . PubMed
- van der Wal E, et al. 2020. Stem Cell Research. 40:101560.. PubMed
- Chen X, et al. 2017. Nat Commun. 10.1038/s41467-017-00687-1. PubMed
- Ramovs V, et al. 2021. Stem Cell Res. 57:102582. PubMed
- Buijsen RAM et al. 2018. Stem cell research. 29:125-128 . PubMed
- Beisang DJ, et al. 2020. Sci Rep. 10:11162. PubMed
- Frederiksen HR et al. 2019. Stem cell research. 1725:03:00 . PubMed
- Bouma MJ, et al. 2019. Stem Cell Research. 41:101654. PubMed
- Frederiksen HR et al. 2019. Stem cell research. 1723:28:00 . PubMed
- Meraviglia V, et al. 2021. Stem Cell Research. 54:102426. PubMed
- van Helden RWJ, et al. 2021. Stem Cell Research. 53:102374. PubMed
- RRID
- AB_1089209 (BioLegend Cat. No. 330401) AB_1089208 (BioLegend Cat. No. 330402)
- Structure
- Carbohydrate epitope on glycosphingolipids
- Distribution
-
Human teratocarcinoma cells (EC), embryonic stem cells (ESC), embryonic germ cells (EG), preimplantation embryos, and erythrocytes; mouse oocytes, early cleaved embryos
- Function
- Regulation of differentiation
- Cell Type
- Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Antigen References
-
1. Kannagi R, et al. 1983. The EMBO J. 2:2355
2. Henderson JK, et al. 2002. Stem Cells. 20:329
3. Brimble SN, et al. 2007. Stem Cells. 25:54
4. Battula VL, et al. 2007. Differentiation. 75:279
- Gene ID
- NA
- UniProt
- View information about SSEA-4 on UniProt.org
