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PE anti-human SSEA-4 Antibody
产品名称:
PE anti-human SSEA-4 Antibody
产品类别:
抗体
产品编号:
330405
产品应用:
330405
[价格]
规格 价格 库存
25tests ¥ 1836 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human embryonic carcinoma cell line 2102Ep
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA); may contain stabilizer.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: ELISA1 and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Kannagi R, et al. 1983. The EMBO J. 2:2355
Product Citations
  1. Nomi K, et al. 2021. Cell Reports. 34(5):108715. PubMed
  2. Hua Y, et al. 2022. Stem Cell Res Ther. 13:223. PubMed
  3. Chung K, et al. 2014. PLoS One. 9:95304. PubMed
  4. Niwa A, et al. 2011. PLoS One. 6:e22261. PubMed
  5. Dor L, et al. 2021. Stem Cell Research. 55:102495. PubMed
  6. Kimura Y, et al. 2020. Stem Cell Research. 72.69236111. PubMed
  7. Zhao M, et al. 2021. Ann Transl Med. 9:1476. PubMed
  8. Wang Y, et al. 2021. Biomaterials. 265:120387. PubMed
  9. Garcia-Montero A, et al. 2016. Blood. 127: 761 - 768. PubMed
  10. Beylina A, et al. 2021. Stem Cell Research. 53:102354. PubMed
  11. Shibata S et al. 2018. Cell reports. 25(6):1668-1679 . PubMed
  12. Klaihmon P, et al. 2021. Stem Cell Research. 55:102472. PubMed
  13. Bi Y, et al. 2020. Cell Reports. 30(11):3917-3931. PubMed
  14. Tanaka Y et al. 2018. Stem cell research. 28:100-104 . PubMed
  15. Tanaka Y et al. 2018. Stem Cell Res. 31:11-15 . PubMed
  16. Zheng Y, et al. 2014. Hum Reprod. 29:2497. PubMed
  17. Pan C, et al. 2019. Int J Mol Med. 44:1629. PubMed
  18. Wei X, et al. 2019. Int J Mol Med. 44:1425. PubMed
  19. Wang G, et al. 2018. Int J Mol Med. 41:791. PubMed
RRID
AB_1089207 (BioLegend Cat. No. 330405) AB_1089206 (BioLegend Cat. No. 330406)

Antigen Details

Structure
Carbohydrate epitope on glycosphingolipids
Distribution

Human teratocarcinoma cells (EC), embryonic stem cells (ESC), embryonic germ cells (EG), preimplantation embryos, and erythrocytes; mouse oocytes, early cleaved embryos

Function
Regulation of differentiation
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Antigen References

1. Kannagi R, et al. 1983. The EMBO J. 2:2355
2. Henderson JK, et al. 2002. Stem Cells. 20:329
3. Brimble SN, et al. 2007. Stem Cells. 25:54
4. Battula VL, et al. 2007. Differentiation. 75:279

Gene ID
NA
UniProt
View information about SSEA-4 on UniProt.org

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