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Alexa Fluor® 647 anti-human SSEA-4 Antibody
产品名称:
Alexa Fluor® 647 anti-human SSEA-4 Antibody
产品类别:
抗体
产品编号:
330407
产品应用:
330407
[价格]
规格 价格 库存
25tests ¥ 1980 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human embryonic carcinoma cell line 2102Ep
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA); may contain stabilizer.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: ELISA1 and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Kannagi R, et al. 1983. The EMBO J. 2:2355
Product Citations
  1. Hohmann MS, et al. 2021. JCI Insight. 6:. PubMed
  2. Li X et al. 2019. Stem cell research. 1726:14:00 . PubMed
  3. Houweling PJ, et al. 2021. Stem Cell Research. 54:102429. PubMed
  4. Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed
  5. Ababneh NA, et al. 2020. Stem Cell Research. 49:102000. PubMed
  6. Gill D, et al. 2022. Elife. 11:. PubMed
  7. D ?pper H, et al. 2020. Stem Cell Research. 72.55486111. PubMed
  8. Nur Patria Y, et al. 2020. Stem Cell Research. 42:101689.. PubMed
  9. Chou S, et al. 2012. Proc Natl Acad Sci U S A. 109:17573. PubMed
  10. Neureiter A et al. 2018. Stem Cell Res. 33:20-24 . PubMed
  11. Schipper L et al. 2018. Stem Cell Res. 33:41-45 . PubMed
  12. Gao Y et al. 2018. Stem cell research. 28:161-164 . PubMed
  13. Ababneh NA, et al. 2021. Stem Cell Research. 54:102358. PubMed
  14. Gagne AL et al. 2018. Stem cell research. 31:157-160 . PubMed
  15. Ababneh NA, et al. 2020. Stem Cell Research. 48:101925. PubMed
  16. Bi X, et al. 2017. EBioMedicine. 10.1016/j.ebiom.2017.03.018. PubMed
  17. Simic MS, et al. 2019. Sci Adv. 5:eaaw0025. PubMed
  18. Steenpass L 2017. Stem Cell Res.. 10.1016/j.scr.2017.07.005. PubMed
  19. Yang W, et al. 2015. PLoS One. 10: 0134995. PubMed
  20. Bi Y, et al. 2020. Cell Reports. 30(11):3917-3931. PubMed
  21. Ababneh NA, et al. 2020. Stem Cell Research. 48:101967. PubMed
  22. Maguire JA et al. 2019. Stem Cell Res. 1723:21:00 . PubMed
  23. Pashos EE et al. 2017. Cell stem cell. 20(4):558-570 . PubMed
  24. Menges J, et al. 2020. Stem Cell Research. 39:101517.. PubMed
  25. Bi Y, et al. 2022. Nat Commun. 13:3147. PubMed
RRID
AB_1089201 (BioLegend Cat. No. 330407) AB_1089200 (BioLegend Cat. No. 330408)

Antigen Details

Structure
Carbohydrate epitope on glycosphingolipids
Distribution

Human teratocarcinoma cells (EC), embryonic stem cells (ESC), embryonic germ cells (EG), preimplantation embryos, and erythrocytes; mouse oocytes, early cleaved embryos

Function
Regulation of differentiation
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Antigen References

1. Kannagi R, et al. 1983. The EMBO J. 2:2355
2. Henderson JK, et al. 2002. Stem Cells. 20:329
3. Brimble SN, et al. 2007. Stem Cells. 25:54
4. Battula VL, et al. 2007. Differentiation. 75:279

Gene ID
NA
UniProt
View information about SSEA-4 on UniProt.org

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