Biotin anti-mouse CD19 Antibody
- 产品名称：
- Biotin anti-mouse CD19 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 101504
- 产品应用：
- 101504
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- mouse CD19+ pre-B cell line 300.19
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation3, and in vitro costimulation (synergistic with anti-IgM) of B cell activation1,2.
- Application References
(PubMed link indicates BioLegend citation) -
- Sato S, et al. 1997. J. Immunol. 158:4662. (Costim)
- Sato S, et al. 1997. P. Natl. Acad. Sci. USA 94:13158. (Costim)
- Krop I, et al. 1996. Eur. J. Immunol. 26:238. (IP)
- Sato S, et al. 1996. J. Immunol. 157:4371.
- Stolk M, et al. 2006. J. Leukoc. Biol. doi:10.1189/jlb.1205739. (FC)
- Shime H, et al. 2012. PNAS. 109:2066. PubMed.
- Product Citations
-
- Becker M, et al. 2017. Proc Natl Acad Sci U S A. 114:5231. PubMed
- Wang H et al. 2017. Developmental cell. 40(6):566-582 . PubMed
- Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed
- Nasarre P, et al. 2021. Cancers (Basel). 13: . PubMed
- Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
- Baja?a S, et al. 2022. iScience. 25:103732. PubMed
- Shime H, et al. 2012. Proc Natl Acad Sci U S A. 109:2066. PubMed
- RRID
- AB_312823 (BioLegend Cat. No. 101504)
- Structure
- Ig superfamily, associates with CD21 and CD81, 95 kD
- Distribution
-
Pro-B cells to mature B cells (during development), follicular dendritic cells
- Function
- Modulates B cell activation and differentiation
- Ligand/Receptor
- CD21, CD81, Leu-13
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Immunology, Costimulatory Molecules
- Molecular Family
- CD Molecules
- Antigen References
-
1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF,et al. 1994. Immunol. Today 15:437.
- Gene ID
- 12478 View all products for this Gene ID
- UniProt
- View information about CD19 on UniProt.org
