Ultra-LEAF™ Purified anti-mouse IFNAR-1 Anti-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1320	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Exogenously expressed murine IFNAR1 extracellular domain

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IP, WB, ELISA, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?L?volume or 100 ?L?of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting^1,2, immunoprecipitation¹, ELISA¹, and blocking^8,9. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 127322)?(Endotoxin <0.01 EU/?g).

Application References (PubMed link indicates BioLegend citation)

Sheehan KC, et al. 2006. J. Interferon Cytokine Res. 26:804. (FC, Block, IP, WB, ELISA)
Dunn GP, et al. 2005. Nat. Immunol. 6:722. (FC, WB)
Miller JC, et al. 2008. J. Immunol. 181:8492. PubMed
Habjan M, et al. 2009. J. Virol. 83:4365. PubMed
Kelly-Scumpia KM, et al. 2010. J. Exp. Med. 207:319. PubMed
Swanson CL, et al. 2010. J. Exp. Med. 207:1485. PubMed
Marshall HD, et al. 2011. J Virol. epub. PubMed
Swann JB, et al. 2007. J. Immunol. 178:7540. (Block)
Jones GW, et al. 2011. FASEB J. 25:409. (Block)
Mitzel DN, et al. 2014. J. Immunol. 192:4273. PubMed

Product Citations

Burke TP, et al. 2020. Nat Microbiol. 0.686111111. PubMed
Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
Kanno T, et al. 2022. Front Immunol. 13:904875. PubMed
Oyler‐Yaniv J et al. 2017. Molecular cell. 66(5):635-647 . PubMed
Kalafati L, et al. 2020. Cell. 183:771. PubMed
Zhao X, et al. 2022. iScience. 25:104690. PubMed

RRID

AB_11150409 (BioLegend Cat. No. 127321) AB_11149116 (BioLegend Cat. No. 127322) AB_2629521 (BioLegend Cat. No. 127323) AB_2629522 (BioLegend Cat. No. 127324) AB_2832460 (BioLegend Cat. No. 127327) AB_2819862 (BioLegend Cat. No. 127328)

Antigen Details

Distribution: IFNAR1 and IFNAR2 are coexpressed on nearly all cells.
Ligand/Receptor: IFN-α/β
Antigen References: 1. Branca AA, et al. 1981. Nature 294:768.
2. Orchansky P, et al. 1984. J. Interferon Res. 4:275.
3. Hemmi S, et al. 1994. Cell 76:803.
4. Novick D, et al. 1994. Cell 77:391.
Gene ID: 15975 View all products for this Gene ID
UniProt: View information about IFNAR-1 on UniProt.org

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