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首页 > 抗体蛋白 > 抗体
APC anti-mouse IFNAR-1 Antibody
产品名称：
APC anti-mouse IFNAR-1 Antibody
产品类别：
抗体
产品编号：
127313
产品应用：
127313
[价格]
规格 价格 库存
25µg ￥ 1704 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Exogenously expressed murine IFNAR1 extracellular domain
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, ELISA1, and blocking8,9. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 127322)?(Endotoxin <0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)
  1. Sheehan KC, et al. 2006. J. Interferon Cytokine Res. 26:804. (FC, Block, IP, WB, ELISA)
  2. Dunn GP, et al. 2005. Nat. Immunol. 6:722. (FC, WB)
  3. Miller JC, et al. 2008. J. Immunol. 181:8492. PubMed
  4. Habjan M, et al. 2009. J. Virol. 83:4365. PubMed
  5. Kelly-Scumpia KM, et al. 2010. J. Exp. Med. 207:319. PubMed
  6. Swanson CL, et al. 2010. J. Exp. Med. 207:1485. PubMed
  7. Marshall HD, et al. 2011. J Virol. epub. PubMed
  8. Swann JB, et al. 2007. J. Immunol. 178:7540. (Block)
  9. Jones GW, et al. 2011. FASEB J. 25:409. (Block)
  10. Mitzel DN, et al. 2014. J. Immunol. 192:4273. PubMed
Product Citations
  1. Diaz-Arevalo D, Kalkum J 2012. Front Microbiol. 22:299. PubMed
  2. Harkins S, et al. 2014. J Virol. 55:5807. PubMed
  3. Kelsey E Sivick et al. 2018. Cell reports. 25(11):3074-3085 . PubMed
  4. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  5. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  6. Yamamoto K, et al. 2021. iScience. 24:103064. PubMed
  7. Ng S, et al. 2022. Sci Rep. 12:14087. PubMed
  8. Shen H, et al. 2022. Nat Commun. 13:5013. PubMed
RRID
AB_2122746 (BioLegend Cat. No. 127313) AB_2122745 (BioLegend Cat. No. 127314)

Antigen Details

Distribution

IFNAR1 and IFNAR2 are coexpressed on nearly all cells.

Ligand/Receptor
IFN-α/β
Antigen References

1. Branca AA, et al. 1981. Nature 294:768.
2. Orchansky P, et al. 1984. J. Interferon Res. 4:275.
3. Hemmi S, et al. 1994. Cell 76:803.
4. Novick D, et al. 1994. Cell 77:391.

Gene ID
15975 View all products for this Gene ID
UniProt
View information about IFNAR-1 on UniProt.org

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