PerCP/Cyanine5.5 anti-mouse FcεRIα A
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse FcεRIα A
- 产品类别：
- 抗体
- 产品编号：
- 134319
- 产品应用：
- 134319
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for relevant formats of this clone) include: depletion2, immunohistochemistry of frozen sections (OCT embedded2).
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Obata K, et al. 2007. Blood 110:913 (FC)
- Sokol CL, et al. 2008. Nat. Immunol. 9:310 (FC, Deplete, IHC)
- Chen J, et al. 2009. J. Biol. Chem.. 284:5763 (FC)
- Product Citations
-
- Oetjen LK et al. 2017. Cell. 171(1):217-228 . PubMed
- Kobayashi T, et al. 2019. Cell. 176:982. PubMed
- Li Y, et al. 2022. BMC Pulm Med. 22:180. PubMed
- Riding AM, et al. 2022. iScience. 25:104660. PubMed
- Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
- Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
- McGinty JW, et al. 2020. Immunity. 52(3):528-541. PubMed
- Yu X, et al. 2016. Blood. 127: 132 - 138. PubMed
- Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
- RRID
- AB_10645508 (BioLegend Cat. No. 134319) AB_10641135 (BioLegend Cat. No. 134320)
- Structure
- A transmembrane protein of Ig super family member
- Distribution
-
Expressed on mast and basophils.
- Function
- Play an important role in triggering IgE-mediated allergic reactions.
- Ligand/Receptor
- IgE
- Cell Type
- Basophils, Mast cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
-
1. Arinobu Y, et al. 2005. P. Natl. Acad. Sci. USA 102:18105.
2. Yamaguchi M, et al. 2001. Int. Immunol. 13:843.
- Gene ID
- 2205 View all products for this Gene ID
- UniProt
- View information about FcepsilonRIalpha on UniProt.org
