Direct-Blot™ HRP anti-Nuclear Pore Complex P-上海雅吉生物科技有限公司

规格	价格	库存
100µL	￥ 4080	1

Product Details

Verified Reactivity

Vertebrate, Xenopus, Yeast

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

The antibody was raised using a nuclear pore complex mixture.

Formulation

This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.

Preparation

The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.

Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:2000-1:10000. The optimal dilution should be determined by titration for each individual assay of interest.

25 ?l and 100 ?l of Direct-Blot? HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), immunoprecipitation (IP) and immunoelectron microscopy (IEM). This antibody has been used successfully with frozen sections.

*Predicted MW = 62 kD

MAb414 is a reliable general purpose monoclonal antibody which recognizes a related family of NPC proteins. This antibody is ideal for studying the morphology and composition of the nucleus and nuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis and meiosis. This antibody is believed to recognize the repeated FXFG repeat sequence in nuceloporins including p62, p152 and p90 and other proteins.

Application References (PubMed link indicates BioLegend citation)

Zheng X, et al. 2012. J. Biol. Chem. 287:38254. (IF) PubMed
Kimura T, et al. 2003. Mol Cell Biol. 23:1304. (IHC) PubMed
Lopez-Soler RI, et al. 2001. J Cell Biol. 154:61. (IF, WB) PubMed
Aris JP, Blobel G. 1989. J Cell Biol. 108:2059. (WB, IP, IF, EM) PubMed
Davis LI, Blobel G. 1987. PNAS USA. 84:7552. (IP, IF) PubMed
Edens LJ, Levy DL. 2014. J. Cell. Biol. 206:473.
Davis LI, Blobel G. 1986. Cell. 45:699.
Blobel G. 1985. PNAS USA 82:8527.

RRID

AB_2629624 (BioLegend Cat. No. 902905)

Antigen Details

Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Nuclear Markers
Antigen References: 1. Yoshimura S, et al. 2013. J. Cell Sci. 126:3141-50.
Gene ID: NA
UniProt: View information about Nuclear Pore Complex Proteins on UniProt.org

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