Alexa Fluor® 594 anti-Nuclear Pore Complex Pro
- 产品名称:
- Alexa Fluor® 594 anti-Nuclear Pore Complex Pro
- 产品类别:
- 抗体
- 产品编号:
- 682202
- 产品应用:
- 682202
- Verified Reactivity
- Vertebrate, Xenopus, Yeast
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- The antibody was raised using a nuclear pore complex mixture.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), immunoprecipitation (IP) and immunoelectron microscopy (IEM). This antibody has been used successfully with frozen sections.
*Predicted MW = 62 kD
MAb414 is a reliable general purpose monoclonal antibody which recognizes a related family of NPC proteins. This antibody is ideal for studying the morphology and composition of the nucleus and nuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis and meiosis.
- Application References
(PubMed link indicates BioLegend citation) -
- Zheng X, et al. 2012. J. Biol. Chem. 287:38254. (IF) PubMed
- Kimura T, et al. 2003. Mol Cell Biol. 23:1304. (IHC) PubMed
- Lopez-Soler RI, et al. 2001. J Cell Biol. 154:61. (IF, WB) PubMed
- Aris JP, Blobel G. 1989. J Cell Biol. 108:2059. (WB, IP, IF, EM) PubMed
- Davis LI, Blobel G. 1987. PNAS USA. 84:7552. (IP, IF) PubMed
- Edens LJ, Levy DL. 2014. J. Cell. Biol. 206:473.
- Davis LI, Blobel G. 1986. Cell. 45:699.?
- Blobel G. 1985. PNAS USA 82:8527.
- Product Citations
-
- Nord MS, et al. 2020. Nucleus. 0.581944444. PubMed
- Dilsaver MR, et al. 2018. Mol Biol Cell. :mbcE18050277. PubMed
- RRID
- AB_2566575 (BioLegend Cat. No. 682202)