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Alexa Fluor® 594 anti-Nuclear Pore Complex Pro
产品名称:
Alexa Fluor® 594 anti-Nuclear Pore Complex Pro
产品类别:
抗体
产品编号:
682202
产品应用:
682202
[价格]
规格 价格 库存
100µg ¥ 4584 1

产品详情

Product Details

Verified Reactivity
Vertebrate, Xenopus, Yeast
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The antibody was raised using a nuclear pore complex mixture.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), immunoprecipitation (IP) and immunoelectron microscopy (IEM). This antibody has been used successfully with frozen sections.

*Predicted MW = 62 kD

MAb414 is a reliable general purpose monoclonal antibody which recognizes a related family of NPC proteins. This antibody is ideal for studying the morphology and composition of the nucleus and nuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis and meiosis.

Application References

(PubMed link indicates BioLegend citation)
  1. Zheng X, et al. 2012. J. Biol. Chem. 287:38254. (IF) PubMed
  2. Kimura T, et al. 2003. Mol Cell Biol. 23:1304. (IHC) PubMed
  3. Lopez-Soler RI, et al. 2001. J Cell Biol. 154:61. (IF, WB) PubMed
  4. Aris JP, Blobel G. 1989. J Cell Biol. 108:2059. (WB, IP, IF, EM) PubMed
  5. Davis LI, Blobel G. 1987. PNAS USA. 84:7552. (IP, IF) PubMed
  6. Edens LJ, Levy DL. 2014. J. Cell. Biol. 206:473.
  7. Davis LI, Blobel G. 1986. Cell. 45:699.?
  8. Blobel G. 1985. PNAS USA 82:8527.
Product Citations
  1. Nord MS, et al. 2020. Nucleus. 0.581944444. PubMed
  2. Dilsaver MR, et al. 2018. Mol Biol Cell. :mbcE18050277. PubMed
RRID
AB_2566575 (BioLegend Cat. No. 682202)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Nuclear Markers
Antigen References
  1. Yoshimura S, et al. 2013. J. Cell Sci. 126:3141-50.
Gene ID
NA
UniProt
View information about Nuclear Pore Complex Proteins on UniProt.org

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