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PE/Cyanine7 anti-human TNF-α Antibody
产品名称：
PE/Cyanine7 anti-human TNF-α Antibody
产品类别：
抗体
产品编号：
502929
产品应用：
502929
[价格]
规格 价格 库存
25tests ￥ 1548 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Cat, Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Sooty Mangabey, Pig
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
E. coli-expressed, recombinant human TNF-α
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA or ELISPOT Detection: The biotinylated MAb11 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT, when used in conjunction with the purified MAb1 antibody (Cat. No. 502802/502804) as the capture antibody.

Flow Cytometry3,5,6,10: The fluorochrome-labeled MAb11 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations.

Additional reported applications (for the relevant formats) include: neutralization1,2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections4 and acetone-fixed frozen tissue sections8, immunocytochemistry7, and immunofluorescence9. The MAb11 antibody can neutralize the bioactivity of natural or recombinant TNF-a.

Note: For testing human TNF-a in serum or plasma, BioLegend's ELISA Max? Sets (Cat. No. 430201 to 430206) are specially developed and recommended. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of human TNF-a bioactivity (Cat. No. 502922).

The Purified MAb1 antibody is useful in neutralization2 and as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MAb11 antibody (Cat. No. 502904/502914) as the detecting antibody.

Clone MAb11 cross-reacts to Cat11

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Rathjen D, et al. 1991. Mol. Immunol. 28:79. (Neut)
  2. Ablamunits V, et al. 2010. Eur. J. Immunol. 40:2891. (Neut)
  3. Enr quez J, et al. 2002. Adv. Perit. Dial. 18:177. (ICFC)
  4. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. (IHC)
  5. Chen H, et al. 2005. J. Immunol. 175:591. (ICFC)
  6. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
  7. Andersson U, et al. 2000. J. Exp. Med. 192:565. (ICC)
  8. Moormann AM, et al. 1999. J. Infect. Dis. 180:1987. (IHC)
  9. Zhao XJ, et al. 2003. J. Immunol. 170:2923. (IF)
  10. Rieger R, et al. 2009. Cancer Gene Ther. 1:53-64. (FC)
  11. Maksaereekul S, et al. 2009. Vaccine. 28:3754 (FC)
Product Citations
  1. Li M, et al. 2021. J Clin Invest. 131:. PubMed
  2. Mayassi T, et al. 2019. Cell. 176:967. PubMed
  3. Viborg N, et al. 2019. Oncoimmunology. 8:e1663107. PubMed
  4. Mastelic-Gavillet B, et al. 2019. J Immunother Cancer. 7:257. PubMed
  5. Pachnio A, et al. 2016. PLoS Pathog. 12: 1005832. PubMed
  6. Chiu Y, et al. 2016. Sci Rep. 6:19227. PubMed
  7. Kim C, et al. 2018. Cell Rep. 25:2148. PubMed
  8. Di Blasi D, et al. 2019. Cell Mol Gastroenterol Hepatol. 0.510416667. PubMed
  9. Marquardt N, et al. 2019. Nat Commun. 10:3841. PubMed
  10. Seo IH, et al. 2021. Immune Netw. 21:e17. PubMed
  11. Pattekar A, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. 11(5):1267-1289. PubMed
  12. Kilpelainen A, et al. 2022. Front Immunol. 13:815041. PubMed
  13. Misra R, et al. 2015. Hum Immunol. 76:329. PubMed
  14. Bradley T et al. 2018. Cell. 175(2):387-399 . PubMed
  15. Saini SK, et al. 2021. Sci Immunol. 6:. PubMed
  16. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  17. Motozono C, et al. 2021. Cell Host Microbe. . PubMed
  18. Wilgenburg B, et al. 2016. Nat Commun. 7:11653. PubMed
  19. Saini SK, et al. 2020. Nat Commun. 4.388888889. PubMed
  20. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  21. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  22. Marzan-Rivera N, et al. 2022. iScience. 25:104764. PubMed
  23. Li L, et al. 2022. JCI Insight. 7:. PubMed
  24. Cimen Bozkus C, et al. 2021. STAR Protoc. 2:100758. PubMed
  25. Ribot J, et al. 2014. J Immunol. 192:2237. PubMed
RRID
AB_2204080 (BioLegend Cat. No. 502929) AB_2204079 (BioLegend Cat. No. 502930)

Antigen Details

Structure
TNF superfamily; dimer/trimer; 17 kD (Mammalian)
Bioactivity
Paracrine/endocrine mediator of inflammatory and immune functions; selectively cytotoxic for transformed cells; chemoattractant
Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Targets
Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
Receptors
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Cell Type
Neutrophils, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Beutler B, et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler B, et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey K, et al. 1993. Crit. Care Med. 21:S415.

Regulation
Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists
Gene ID
7124 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

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