Ultra-LEAF™ Purified anti-human TNF-α -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2448	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

E. coli-expressed, recombinant human TNF-α

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

ELISA Capture - Quality tested
ELISPOT Capture, Neut, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISPOT capture antibody, a concentration range of 0.25 - 1.0 ?g/ml is recommended. For ELISA capture applications, a concentration range of 1.0 - 4.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of TNF-α recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture: The purified MAb1 antibody is useful in neutralization² and as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MAb11 antibody (Cat. No. 502904/502914) as the detecting antibody. The Ultra-LEAF? purified antibody is suggested for ELISPOT capture.

Application References (PubMed link indicates BioLegend citation)

Rathjen, D., et al. 1991. Mol. Immunol. 28:79.
Verschoor CP, et al. 2015. Mol. Immunol. 65:148. (Neut) Pubmed
Wyant, T. L., et al. 1999. Infect. Immun. 67:1338.
Nichols, J. E., et al. 2001. J. Virol. 73:5921.
Ivanov,VN.,et al. 1999. J. Biol Chem. 274:14079. PubMed
Varadarajan N, et al. 2012. PNAS. 109:3885. PubMed.

Product Citations

Benjamin Kr?mer, et al. 2021. Immunity.. Online ahead of print. PubMed
OConnor RA, et al. 2021. OncoImmunology. 10(1):1940675. PubMed

RRID

AB_2814397 (BioLegend Cat. No. 502805) AB_2814398 (BioLegend Cat. No. 502806)

Antigen Details

Structure: TNF superfamily; dimer/trimer; 52 kD (Mammalian)
Bioactivity: Paracrine/endocrine mediator of inflammatory and immune functions; selectively cytotoxic for transformed cells; chemoattractant
Cell Sources: Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Targets: Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
Receptors: TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Beutler, B., et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler, B., et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey, K., et al. 1993. Crit. Care Med. 21:S415.
Regulation: Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists,
Gene ID: 7124 View all products for this Gene ID
UniProt: View information about TNF-alpha on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们