PE/Cyanine7 anti-mouse CD66a (CEACAM1a) Antibody
- 产品名称：
- PE/Cyanine7 anti-mouse CD66a (CEACAM1a) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 134515
- 产品应用：
- 134515
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- SJL/J mice immunized with deoxycholate extracted proteins from BALB/c intestinal brush border membranes.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Mab-CC1 clone recognizes the MHV binding domain of CD66a2. Pretreatment of murine fibroblasts with Mab-CC1 prevented binding of MHV-A59 and protected cells from infection with MHV-A592. SJL/J mice are resistant to MHV as they express an alternative allele, clone Mab-CC1 does not react against SJL/J cells2.
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Turner BC, et al. 2004. J. Virol. 78 (10):5486
- Williams RK, et al. 1990. J. Virol. 64:3817
- Dveksler GS, et al. 1993. Proc. Natl. Acad. Sci. USA. 90:1716
- RRID
- AB_2632802 (BioLegend Cat. No. 134515) AB_2632803 (BioLegend Cat. No. 134516)
- Structure
- A glycoprotein of the immunoglobulin (Ig) superfamily and the carcinoembryonic antigen family
- Distribution
-
CEACAM1a is abundantly expressed on B cells, granulocytes, some NK cells, monocytes, DCs, and NK cells. It is found on epithelial and endothelial cells.
- Function
- CEACAM1a is involved in cell adhesion, tumor growth, and viral infection. It regulates on BCR activation of B lymphocytes.
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Epithelial cells, Granulocytes, Monocytes, NK cells
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Nakagaki K,?et al.?2005.?J. Virol.?79(10):6102
2. Greicius G?et al.?2003.?J. Leukoc. Biol.?74(1):126
3. Hemmila E?et al.?2004.?J. Virol.?78(18):10156
- Gene ID
- 26365 View all products for this Gene ID
- UniProt
- View information about CD66a on UniProt.org
