Brilliant Violet 650™ anti-mouse CD66a (CEAC
- 产品名称：
- Brilliant Violet 650™ anti-mouse CD66a (CEAC
- 产品类别：
- 抗体
- 产品编号：
- 134529
- 产品应用：
- 134529
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- SJL/J mice immunized with deoxycholate extracted proteins from BALB/c intestinal brush border membranes.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650? under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC -?Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 650? excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650? is a trademark of Sirigen Group Ltd.?
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Mab-CC1 clone recognizes the MHV binding domain of CD66a2. Pretreatment of murine fibroblasts with Mab-CC1 prevented binding of MHV-A59 and protected cells from infection with MHV-A592. SJL/J mice are resistant to MHV as they express an alternative allele, clone Mab-CC1 does not react against SJL/J cells2.
- Application References
(PubMed link indicates BioLegend citation) -
- Turner BC, et al. 2004. J. Virol. 78 (10):5486
- Williams RK, et al. 1990. J. Virol. 64:3817
- Dveksler GS, et al. 1993. Proc. Natl. Acad. Sci. USA. 90:1716
- RRID
- AB_2687362 (BioLegend Cat. No. 134529)
- Structure
- A glycoprotein of the immunoglobulin (Ig) superfamily and the carcinoembryonic antigen family
- Distribution
-
CEACAM1a is abundantly expressed on B cells, granulocytes, some NK cells, monocytes, DCs, and NK cells. It is found on epithelial and endothelial cells.
- Function
- CEACAM1a is involved in cell adhesion, tumor growth, and viral infection. It regulates on BCR activation of B lymphocytes.
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Epithelial cells, Granulocytes, Monocytes, NK cells
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Nakagaki K,?et al.?2005.?J. Virol.?79(10):6102
2. Greicius G?et al.?2003.?J. Leukoc. Biol.?74(1):126
3. Hemmila E?et al.?2004.?J. Virol.?78(18):10156
- Gene ID
- 26365 View all products for this Gene ID
- UniProt
- View information about CD66a on UniProt.org
