APC/Fire™ 750 anti-mouse CD66a (CEACAM1a) An-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3960	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

SJL/J mice immunized with deoxycholate extracted proteins from BALB/c intestinal brush border membranes.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with APC/Fire? 750 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire? 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Application Notes

Mab-CC1 clone recognizes the MHV binding domain of CD66a². Pretreatment of murine fibroblasts with Mab-CC1 prevented binding of MHV-A59 and protected cells from infection with MHV-A59². SJL/J mice are resistant to MHV as they express an alternative allele, clone Mab-CC1 does not react against SJL/J cells².

Application References (PubMed link indicates BioLegend citation)

Turner BC, et al. 2004. J. Virol. 78 (10):5486
Williams RK, et al. 1990. J. Virol. 64:3817
Dveksler GS, et al. 1993. Proc. Natl. Acad. Sci. USA. 90:1716

RRID

AB_2632807 (BioLegend Cat. No. 134520)

Antigen Details

Structure: A glycoprotein of the immunoglobulin (Ig) superfamily and the carcinoembryonic antigen family
Distribution: CEACAM1a is abundantly expressed on B cells, granulocytes, some NK cells, monocytes, DCs, and NK cells. It is found on epithelial and endothelial cells.
Function: CEACAM1a is involved in cell adhesion, tumor growth, and viral infection. It regulates on BCR activation of B lymphocytes.
Cell Type: B cells, Dendritic cells, Endothelial cells, Epithelial cells, Granulocytes, Monocytes, NK cells
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Nakagaki K,?et al.?2005.?J. Virol.?79(10):6102
2. Greicius G?et al.?2003.?J. Leukoc. Biol.?74(1):126
3. Hemmila E?et al.?2004.?J. Virol.?78(18):10156
Gene ID: 26365 View all products for this Gene ID
UniProt: View information about CD66a on UniProt.org

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