Ultra-LEAF™ Purified anti-human CD158d (KIR2
- 产品名称：
- Ultra-LEAF™ Purified anti-human CD158d (KIR2
- 产品类别：
- 抗体
- 产品编号：
- 347009
- 产品应用：
- 347009
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- NK3.3 cells and KIR2DL4-Ig fusion protein
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Activ - Reported in literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by?immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106?cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
KIR2DL4 expression in NK cells is highly localized to the endosomes3. mAb 33 can be internalized by NK cells by endocytosis at 37°C3, and induces NK cell cytotoxicity1,2. Based on in-house observations, we suggest incubating for 45 - 60 minutes at room temperature with clone mAb 33 to observe better CD158d staining.
This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).
- Application References
(PubMed link indicates BioLegend citation) -
- Rajagopalan S, et al. 2001. J. Immunol. 167:1877. (FC)
- Goodridge JP, et al. 2003. J. Immunol. 171:1768. (FC)
- Rajagopalan S, et al. 2006. PLoS Biol. 4:e9.
- RRID
- AB_2894481 (BioLegend Cat. No. 347009) AB_2894481 (BioLegend Cat. No. 347010)
- Structure
- Contains two Ig-like extracellular domains, 45-50 kD
- Distribution
-
On CD56high NK cells and cultured CD56dim NK cells
- Ligand/Receptor
- HLA-G
- Cell Type
- NK cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Rajagopalan S, et al. 2006. PLoS Biol. 4:e9.
2. Goodridge JP, et al. 2007. Eur. J. Immunol. 37:199.
- Gene ID
- 3805 View all products for this Gene ID
- UniProt
- View information about CD158d on UniProt.org
