APC anti-human CD158d (KIR2DL4) Antibody
- 产品名称：
- APC anti-human CD158d (KIR2DL4) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 347007
- 产品应用：
- 347007
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1848 |
1 |
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- NK3.3 cells and KIR2DL4-Ig fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
KIR2DL4 expression in NK cells is highly localized to the endosomes3. mAb 33 can be internalized by NK cells by endocytosis at 37°C3, and induces NK cell cytotoxicity1,2. Based on in-house observations, we suggest incubating for 45 - 60 minutes at room temperature with clone mAb 33 to observe better CD158d staining.
This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).
- Application References
(PubMed link indicates BioLegend citation) -
- Rajagopalan S, et al. 2001. J. Immunol. 167:1877. (FC)
- Goodridge JP, et al. 2003. J. Immunol. 171:1768. (FC)
- Rajagopalan S, et al. 2006. PLoS Biol. 4:e9.
- Product Citations
-
- Brusilovsky M, et al. 2013. J Immunol. 191:5256. PubMed
- Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
- RRID
- AB_2249479 (BioLegend Cat. No. 347007) AB_2130691 (BioLegend Cat. No. 347008)
- Structure
- Contains two Ig-like extracellular domains, 45-50 kD
- Distribution
-
On CD56high NK cells and cultured CD56dim NK cells
- Ligand/Receptor
- HLA-G
- Cell Type
- NK cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Rajagopalan S, et al. 2006. PLoS Biol. 4:e9.
2. Goodridge JP, et al. 2007. Eur. J. Immunol. 37:199.
- Gene ID
- 3805 View all products for this Gene ID
- UniProt
- View information about CD158d on UniProt.org
