Purified anti-human CD14 Antibody
- 产品名称：
- Purified anti-human CD14 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 301801
- 产品应用：
- 301801
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Capuchin Monkey, Cow, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Dog, Pigtailed Macaque, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length human CD14 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF? - Verified
Block, IHC-F, PG - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 301861 and 301862).
- Application References
(PubMed link indicates BioLegend citation) -
- McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
- Williams KC, et al. 2001. J. Exp. Med. 193:905.
- Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
- Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- Zizzo G, et al. 2012. J Immunol. 189:3508. PubMed
- Humblet–Baron S, et al. 2019. J Allergy Clin Immunol. 143:2215. PubMed
- Kaushal A, et al. 2021. Blood Cancer Discov. 2:600. PubMed
- Gadalla R, et al. 2022. STAR Protoc. 3:101643. PubMed
- Melo-Gonzalez F, et al. 2018. J Biol Chem. 293:8543. PubMed
- Korn MA, et al. 2020. J Immunol. 205:2595. PubMed
- Ortiz AM, et al. 2018. Nat Med. 24:1313. PubMed
- Nguyen LT, et al. 2019. Cancer Immunol Immunother. 68:773. PubMed
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- Tal MC, et al. 2020. MBio. 11:00. PubMed
- Winkler M, et al. 2017. PLoS One.. 10.1371/journal.pone.0182427. PubMed
- Coelho CH, et al. 2020. JCI Insight. 5:. PubMed
- Tian Y, et al. 2019. Cell Rep. 29:4482. PubMed
- Raffray L, et al. 2020. Immunology. 159:88. PubMed
- Li H, et al. 2020. Front Immunol. 11:1777. PubMed
- Kong R, et al. 2019. Cell. 178:567. PubMed
- Olin A, et al. 2018. Cell. 174:1277. PubMed
- Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
- Wastyk HC, et al. 2021. Cell. 184(16):4137-4153.e14. PubMed
- Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed
- Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed
- Auladell M, et al. 2019. Clin Transl Immunology. 8:e01090. PubMed
- Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
- Zhao J, et al. 2017. Sci Immunol. 2:eaan5400. PubMed
- Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
- Ehinger E, et al. 2021. Cardiovasc Res. 117:1166. PubMed
- Henrick BM, et al. 2021. Cell. . PubMed
- Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
- Schaut RG, et al. 2019. Sci Rep. 4.820138889. PubMed
- Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
- Schwabenland M, et al. 2021. Immunity. . PubMed
- Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
- De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed
- Oda H, et al. 2019. Front Immunol. 10:479. PubMed
- Zimmerman MG, et al. 2018. Cell Host Microbe. 24:731. PubMed
- Lakshmikanth T, et al. 2020. Cell Reports. 32(3):107923. PubMed
- Iwamoto S, et al. 2007. J Immunol. 179:1449. PubMed
- Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
- Sung JA, et al. 2018. Mol Ther. 26:2496. PubMed
- Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed
- Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
- Roberts JT, et al. 2020. Mol Cell Proteomics. 19:362. PubMed
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- Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
- RRID
- AB_314183 (BioLegend Cat. No. 301801) AB_314184 (BioLegend Cat. No. 301802)
- Structure
- GPI-linked membrane glycoprotein, 53-55 kD
- Distribution
-
Monocytes, macrophages, granulocytes (low)
- Function
- LPS receptor, clearance of Gram-negative pathogens
- Ligand/Receptor
- LPS
- Cell Type
- Granulocytes, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.
- Gene ID
- 929 View all products for this Gene ID
- UniProt
- View information about CD14 on UniProt.org
