PE/Cyanine7 anti-human CD14 Antibody
- 产品名称：
- PE/Cyanine7 anti-human CD14 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 301813
- 产品应用：
- 301813
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1716 |
1 |
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Capuchin Monkey, Cow, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Dog, Pigtailed Macaque, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length human CD14 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 301861 and 301862).
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
- Williams KC, et al. 2001. J. Exp. Med. 193:905.
- Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
- Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
- Zizzo G, et al. 2012. J Immunol. 189:3508. PubMed
- Rizzo MD, et al. 2019. J Pharmacol Exp Ther. 371:191. PubMed
- Schimek V, et al. 2022. Cell Death Dis. 13:113. PubMed
- Subhi Y,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5242-5250. PubMed
- Cross S, et al. 2011. J Immunol. 187:5015. PubMed
- Cines DB, et al. 2020. Blood. 135:2085. PubMed
- Pellett Madan R, et al. 2020. Clin Transplant. 34:e14021. PubMed
- Huang X, et al. 2020. Clin Transl Med. 10:e41. PubMed
- Szentkereszty M, et al. 2020. Pathol Oncol Res. 26:1117. PubMed
- Spencer A, et al. 2014. PLoS One. 9:100538. PubMed
- Bruveris FF, et al. 2020. Development. 147:00:00. PubMed
- Crespo-Lessmann A, et al. 2017. J Asthma Allergy. 10:269. PubMed
- Rizzo MD, et al. 2018. AIDS. 32:419. PubMed
- Sagebiel AF, et al. 2019. Nat Commun. 10:975. PubMed
- Andreano E, et al. 2021. Nature. Online ahead of print. PubMed
- Rizzo MD, et al. 2019. J Neuroimmunol. 333:576969. PubMed
- Matlung HL et al. 2018. Cell reports. 23(13):3946-3959 . PubMed
- Andreano E, et al. 2021. Cell. 184(7):1821-1835.e16. PubMed
- RRID
- AB_389352 (BioLegend Cat. No. 301813) AB_389353 (BioLegend Cat. No. 301814)
- Structure
- GPI-linked membrane glycoprotein, 53-55 kD
- Distribution
-
Monocytes, macrophages, granulocytes (low)
- Function
- LPS receptor, clearance of Gram-negative pathogens
- Ligand/Receptor
- LPS
- Cell Type
- Granulocytes, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.
- Gene ID
- 929 View all products for this Gene ID
- UniProt
- View information about CD14 on UniProt.org
