PE anti-human CD14 Antibody
- 产品名称：
- PE anti-human CD14 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 301850
- 产品应用：
- 301850
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Capuchin Monkey, Cow, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Dog, Pigtailed Macaque, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length human CD14 protein
- Formulation
- ?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). - Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- ?g sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 301861 and 301862).
- Application References
(PubMed link indicates BioLegend citation) -
- McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
- Williams KC, et al. 2001. J. Exp. Med. 193:905.
- Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
- Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- Babania O, et al. 2021. J Clin Lab Anal. 35:e23984. PubMed
- Dhariwala MO, et al. 2020. Cell Rep Med. 1:100132. PubMed
- Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed
- Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
- Nemati S, et al. 2019. J Tissue Eng Regen Med. 13:25. PubMed
- Kuwada K, et al. 2018. J Exp Clin Cancer Res. 37:307. PubMed
- O'Neill MB, et al. 2021. Front Immunol. 12:768189. PubMed
- Bekere I, et al. 2021. PLoS Pathog. 17:e1010074. PubMed
- Li Y, et al. 2022. Cell Rep. 38:110284. PubMed
- Eriksson E, et al. 2016. Gene Ther. 10.1038/gt.2016.80. PubMed
- Kho D, et al. 2017. PLoS One. 10.1371/journal.pone.0180267. PubMed
- Zhang D, et al. 2020. Oncoimmunology. 9:1744921. PubMed
- Luo W, et al. 2022. Front Immunol. 13:816761. PubMed
- Wood MP, et al. 2020. J Infect Dis. 222:44. PubMed
- Tatematsu M, et al. 2016. J Immunol. 196: 3865 - 3876. PubMed
- Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
- Walter F, et al. 2020. PLoS One. 15:e0239369. PubMed
- Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
- Yang C, et al. 2021. J Invest Dermatol. 810:141. PubMed
- Gomez I, et al. 2020. Nat Commun. 11:214. PubMed
- Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed
- Gopinath A, et al. 2022. NPJ Parkinsons Dis. 8:72. PubMed
- Huang N, et al. 2020. Genome Biol. 1.03125. PubMed
- Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
- Karantanos T, et al. 2022. Sci Adv. 8:eabl8952. PubMed
- Weitz JR, et al. 2020. Diabetes. 69:1206. PubMed
- Miyamoto T, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
- Fisher J, et al. 2014. Clin Cancer Res. 20:5720. PubMed
- Ravenhill BJ, et al. 2020. Sci Rep. 10:4560. PubMed
- Zimmerman MG, et al. 2018. Cell Host Microbe. 24:731. PubMed
- Luvanda MK, et al. 2021. J Fungi (Basel). 7:. PubMed
- Foster G, et al. 2015. J Immunol. 195: 5380 - 5392. PubMed
- Lavaert M, et al. 2020. Immunity. 52(6):1088-1104. PubMed
- Reitinger C, et al. 2022. Front Immunol. 13:970290. PubMed
- Foote JR, et al. 2019. Front Immunol. 10:188. PubMed
- Lin HT, et al. 2019. Int J Mol Med. 43:1085. PubMed
- Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
- Wang X, et al. 2021. Immunity. 54(6):1123-1136.e8. PubMed
- Hamdan F, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(8):. PubMed
- Crippa S, et al. 2019. J Clin Invest. 129:1566. PubMed
- Bailey AL, et al. 2020. bioRxiv. . PubMed
- Srisuwan S, et al. 2014. PLoS One. 9:110321. PubMed
- RRID
- AB_314187 (BioLegend Cat. No. 301805) AB_314188 (BioLegend Cat. No. 301806) AB_2564138 (BioLegend Cat. No. 301850)
- Structure
- GPI-linked membrane glycoprotein, 53-55 kD
- Distribution
-
Monocytes, macrophages, granulocytes (low)
- Function
- LPS receptor, clearance of Gram-negative pathogens
- Ligand/Receptor
- LPS
- Cell Type
- Granulocytes, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.
- Gene ID
- 929 View all products for this Gene ID
- UniProt
- View information about CD14 on UniProt.org
