Pacific Blue™ anti-human CD14 Antibody
- 产品名称：
- Pacific Blue™ anti-human CD14 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 301816
- 产品应用：
- 301816
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Capuchin Monkey, Cow, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Dog, Pigtailed Macaque, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length human CD14 protein
- Formulation
- test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
?g sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. - Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- test size: lot-specific; ?g sizes: 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
For ?g sizes, the suggested use of this reagent is ≤ 2.0 ?g per 106 cells in 100 ?l volume or 100 ?l of whole blood.
It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 301861 and 301862).
- Application References
(PubMed link indicates BioLegend citation) -
- McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
- Williams KC, et al. 2001. J. Exp. Med. 193:905.
- Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
- Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- Ilinykh PA, et al. 2020. Cell Host & Microbe. 27(6):976-991. PubMed
- Ho CH, et al. 2021. Arthritis Res Ther. 23:199. PubMed
- Greenshields-Watson A, et al. 2020. Cell Rep. 32:107885. PubMed
- Schmitz T, et al. 2021. J Reprod Immunol. 148:103424. PubMed
- Kerkman PF, Kempers AC 2016. Ann Rheum Dis. 75(12):2201-2207. PubMed
- Li H, et al. 2015. J Infect Dis. 211:1717. PubMed
- Dolton G, et al. 2022. Cell. 185:2936. PubMed
- Alkanani A, et al. 2012. Diabetes. 61:2525. PubMed
- Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed
- Ciarlo E, et al. 2016. Sci Rep. 6:37944. PubMed
- Santer D, et al. 2010. J Immunol. 185:4738. PubMed
- van Kempen TS, et al. 2020. Eur J Immunol. 50:1209. PubMed
- Bernardes JP, et al. 2020. Immunity. 53:1296. PubMed
- Hazeldine J, et al. 2017. PLoS Med. 10.1371/journal.pmed.1002338. PubMed
- Bradley T et al. 2018. Cell. 175(2):387-399 . PubMed
- Tungatt K, et al. 2015. J Immunol. 194:463. PubMed
- Crane A, et al. 2018. Alzheimers Dement. 14:243. PubMed
- Lee J, et al. 2017. PLoS One.. 10.1371/journal.pone.0183594. PubMed
- Rius C, et al. 2018. J Immunol. 200:2263. PubMed
- Holland C, et al. 2015. J Immunol. 195: 5827 - 5836. PubMed
- Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
- Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
- Longino NV, et al. 2019. Cancer Immunol Res. 7:1727. PubMed
- RRID
- AB_493164 (BioLegend Cat. No. 301816) AB_2275670 (BioLegend Cat. No. 301828) AB_493163 (BioLegend Cat. No. 301815)
- Structure
- GPI-linked membrane glycoprotein, 53-55 kD
- Distribution
-
Monocytes, macrophages, granulocytes (low)
- Function
- LPS receptor, clearance of Gram-negative pathogens
- Ligand/Receptor
- LPS
- Cell Type
- Granulocytes, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.
- Gene ID
- 929 View all products for this Gene ID
- UniProt
- View information about CD14 on UniProt.org
