GMP Pacific Blue™ anti-human CD14 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100tests	￥ 4032	1

Product Details

Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Full-length human CD14 protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Pacific Blue? under optimal conditions.

Concentration

200 ?g/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.?

Excitation Laser

Violet Laser (405 nm)

Application Notes

The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation⁴, and immunofluorescence microscopy⁵. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 301861 and 301862).

Application References (PubMed link indicates BioLegend citation)

McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
Williams KC, et al. 2001. J. Exp. Med. 193:905.
Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)

RRID

AB_2924520 (BioLegend Cat. No. 260172)

Disclaimer

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

Batch-to-batch consistency
Material traceability
Documented procedures
Documented employee training
Equipment maintenance and monitoring records
Lot-specific certificates of analysis
Quality audits per ISO 13485:2016
QA review of released products

Antigen Details

Structure: GPI-linked membrane glycoprotein, 53-55 kD
Distribution: Monocytes, macrophages, granulocytes (low)
Function: LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor: LPS
Cell Type: Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.
Gene ID: 929 View all products for this Gene ID
UniProt: View information about CD14 on UniProt.org

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