GMP APC anti-human CD14 Antibody
- 产品名称：
- GMP APC anti-human CD14 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 260056
- 产品应用：
- 260056
[价格]
| 规格 |
价格 |
库存 |
| 100tests |
￥ 4164 |
1 |
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length human CD14 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.2% (w/v) BSA (origin USA) and a stabilizer.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- 200 ?g/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 301861 and 301862).
- Application References
(PubMed link indicates BioLegend citation) -
- McMichael A,?et al.?1987. Leucocyte Typing III. Oxford University Press. New York.
- Knapp W,?et al.?Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
- Schlossman S,?et al.?Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Power CP,?et al.?2004.?J. Immunol.?173:5229. (Block)
- Williams KC,?et al.?2001.?J. Exp. Med.?193:905.
- Iwamoto S,?et al.?2007.?J. Immunol.?179:1449. (FC)?PubMed
- Santer DM,?et al.?2010.?J. Immunol.?485:4739.?PubMed
- Yoshino N,?et al.?2000.?Exp. Anim. (Tokyo)?49:97. (FC)
- Zizzo G,?et al.?2012.?J. Immunol.?189:3508.?PubMed
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- RRID
- AB_2910363 (BioLegend Cat. No. 260056)
- Disclaimer
GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:
- Batch-to-batch consistency
- Material traceability
- Documented procedures
- Documented employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016
- QA review of released products
- Structure
- GPI-linked membrane glycoprotein, 53-55 kD
- Distribution
-
Monocytes, macrophages, granulocytes (low)
- Function
- LPS receptor, clearance of Gram-negative pathogens
- Ligand/Receptor
- LPS
- Cell Type
- Granulocytes, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Stocks S,?et al.?1990.?Biochem. J.?268:275.
2. Wright S,?et al.?1990.?Science?249:1434.
- Gene ID
- 929 View all products for this Gene ID
- UniProt
- View information about CD14 on UniProt.org
