APC/Cyanine7 anti-human CD14 Antibody
- 产品名称：
- APC/Cyanine7 anti-human CD14 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 301819
- 产品应用：
- 301819
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1716 |
1 |
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Capuchin Monkey, Cow, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Dog, Pigtailed Macaque, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length human CD14 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 301861 and 301862).
- Additional Product Notes
- BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
- Williams KC, et al. 2001. J. Exp. Med. 193:905.
- Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
- Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- Pozzetto B, et al. 2021. Nature. 600:701. PubMed
- Schmitz T, et al. 2021. J Reprod Immunol. 148:103424. PubMed
- Prete G, et al. 2016. Antimicrob Agents Chemother. 60: 1560 - 1572. PubMed
- Gajdasik DW, et al. 2020. Nat Commun. 2.834027778. PubMed
- Zhang B, et al. 2022. J Exp Med. 219:. PubMed
- Lysenko V, et al. 2020. Blood Adv. 4:2477. PubMed
- Krauss PL, et al. 2021. Front Immunol. 12:730672. PubMed
- Glaros V, et al. 2021. Immunity. 54:2005. PubMed
- Gleason M, et al. 2014. Blood. 123:3016. PubMed
- Kuehn C, et al. 2014. Pathol Biol (Paris). 62:354. PubMed
- Muto S, et al. 2021. Oncol Lett. 21:203. PubMed
- Sureshchandra S, et al. 2021. iScience. 24:102690. PubMed
- Goods BA, et al. 2021. JCI Insight. 6: . PubMed
- Prete G, et al. 2014. Antimicrob Agents Chemother. 58:6790. PubMed
- Lee JH, et al. 2021. NPJ Vaccines. 6:113. PubMed
- Herati RS, et al. 2021. Cell Reports Medicine. 2(5):100262. PubMed
- Zabaleta N, et al. 2021. Cell Host Microbe. :. PubMed
- Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
- Janela B, et al. 2019. Immunity. 50:1069. PubMed
- Liu W, et al. 2022. Bio Protoc. 12:e4285. PubMed
- Prete G, et al. 2012. J Virol. 86:3152. PubMed
- Meckiff BJ, et al. 2020. Cell. 183(5):1340-1353.e16. PubMed
- Shemesh A, et al. 2022. J Exp Med. 219:. PubMed
- Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
- Messerer DAC, et al. 2020. Front Immunol. 11:571992. PubMed
- Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
- R?nnberg E, et al. 2019. Front Immunol. 1.361805556. PubMed
- Clayton KL, et al. 2021. Cell Host Microbe. 29(3):435-447.e9. PubMed
- Askenase MH, et al. 2021. Sci Immunol. 6:. PubMed
- Leboux RJT, et al. 2022. Front Allergy. 2:642788. PubMed
- Inglis H, et al. 2015. J Vis Exp. 97: 52484. PubMed
- Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
- RRID
- AB_493694 (BioLegend Cat. No. 301819) AB_493695 (BioLegend Cat. No. 301820)
- Structure
- GPI-linked membrane glycoprotein, 53-55 kD
- Distribution
-
Monocytes, macrophages, granulocytes (low)
- Function
- LPS receptor, clearance of Gram-negative pathogens
- Ligand/Receptor
- LPS
- Cell Type
- Granulocytes, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.
- Gene ID
- 929 View all products for this Gene ID
- UniProt
- View information about CD14 on UniProt.org
