APC anti-human CD14 Antibody
- 产品名称：
- APC anti-human CD14 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 301807
- 产品应用：
- 301807
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Capuchin Monkey, Cow, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Dog, Pigtailed Macaque, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length human CD14 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 301861 and 301862).
- Application References
(PubMed link indicates BioLegend citation) -
- McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
- Williams KC, et al. 2001. J. Exp. Med. 193:905.
- Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
- Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- Zhu ZY, et al. 2022. Front Immunol. 12:781087. PubMed
- Lopes MG, et al. 2021. J Dairy Sci. 104:9340. PubMed
- Mahmud FJ, et al. 2020. J Neuroinflammation. 0.897916667. PubMed
- Downes DJ, et al. 2021. Nat Genet. 53:1606. PubMed
- Woolsey C, et al. 2020. Scientific Reports. 10(1):3071.. PubMed
- Alharthi AS, et al. 2019. J Dairy Sci. 102:10599. PubMed
- Guo Z, et al. 2022. NPJ Precis Oncol. 6:34. PubMed
- Ferrari M, et al. 2021. J Virol. 95:e0068521. PubMed
- Offersen R, et al. 2016. J Virol. 90: 4441 - 4453. PubMed
- Woolsey C et al. 2019. Cell Rep. 28(12):3032-3046 . PubMed
- Kedage V, et al. 2019. MAbs. 12:1685832. PubMed
- Yokoyama K, et al. 2022. Front Immunol. 13:1016914. PubMed
- Jankeel A, et al. 2020. J Virol. :94. PubMed
- Constantinescu-Bercu A, et al. 2020. eLife. 9:00. PubMed
- Spildrejorde M, et al. 2014. Physiol Genomics. 46:512. PubMed
- Mu Z, et al. 2021. Genome Biol. 122:22. PubMed
- Takele Y, et al. 2022. PLoS Negl Trop Dis. 16:e0010544. PubMed
- Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed
- Claus C, et al. 2021. Pharmaceutics. 13:. PubMed
- Wegner J, et al. 2021. Sci Rep. 11:14983. PubMed
- Broeren MGA, et al. 2018. ALTEX. 36:18:00. PubMed
- da Rocha Sobrinho HM, et al. 2021. Journal of Neuroscience Research. :. PubMed
- Powell EJ, et al. 2018. J Dairy Sci. 101:8182. PubMed
- Ferguson Bennit HR, et al. 2017. Blood Lymphat Cancer. 7:9. PubMed
- Weiss R, et al. 2021. Cells. 10:. PubMed
- Chen Y, et al. 2021. Front Mol Biosci. 8:777370. PubMed
- Zhou Z, et al. 2018. J Dairy Sci. 101:10374. PubMed
- T?htinen S, et al. 2022. Nat Immunol. 23:532. PubMed
- Kiers D et al. 2018. EBioMedicine. 33:144-156 . PubMed
- Lavaert M, et al. 2020. Immunity. 52(6):1088-1104. PubMed
- Duncan CJA, et al. 2022. J Exp Med. 219:. PubMed
- Bade RM, et al. 2021. Molecular Oncology. . PubMed
- Miksiunas R, et al. 2020. Int J Mol Sci. 21:00. PubMed
- Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed
- Wang H, et al. 2017. J Neurochem. 141:347. PubMed
- Thomas BL, et al. 2022. J Dairy Sci. 105:2256. PubMed
- Magg T, et al. 2021. Sci Immunol. 6:. PubMed
- RRID
- AB_314189 (BioLegend Cat. No. 301807) AB_314190 (BioLegend Cat. No. 301808)
- Structure
- GPI-linked membrane glycoprotein, 53-55 kD
- Distribution
-
Monocytes, macrophages, granulocytes (low)
- Function
- LPS receptor, clearance of Gram-negative pathogens
- Ligand/Receptor
- LPS
- Cell Type
- Granulocytes, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.
- Gene ID
- 929 View all products for this Gene ID
- UniProt
- View information about CD14 on UniProt.org
